We narrowed to 23,817 results for: Spr

1. pEPOR0SP0020

   Plasmid

   #117531

   Purpose

   Level0 Golden Gate part: CDS, SaCas9

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SaCas9 (with stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMVP-Cas9C-P2A-turboGFP

   Plasmid

   #80935

   Purpose

   Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. OA-1050G (white)

   Plasmid

   #132420

   Purpose

   express arrays of gRNA targeting White under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   white gRNA array
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTX179

   Plasmid

   #89261

   Purpose

   Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC155-pCR8-sgExpression

   Plasmid

   #49045

   Purpose

   U6-driven sgRNA expressing cassette on a gateway donor vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. OA-1050J (GFP)

   Plasmid

   #133304

   Purpose

   express arrays of gRNA targeting GFP under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   GFP gRNA array
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMB62

   Plasmid

   #47944

   Purpose

   Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

   Expression

   Worm

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330A_FokI-1x2

   Plasmid

   #63602

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR1054

   Plasmid

   #71822

   Purpose

   Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL690

    Plasmid

    #47753

    Purpose

    Expresses dCas9-VP64 fusion

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCD017

    Plasmid

    #177322

    Purpose

    dCas9 expression

    Depositor

    James Carothers

    Article

    Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9–mKate2ps–CgRNA

    Plasmid

    #64955

    Purpose

    Control gRNA (GTCAAGGCACTCTTGCCTA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLH-stsgRNA2.1

    Plasmid

    #64117

    Purpose

    Vector for expression of St1 sgRNA2.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9–mKate2ps–T1gRNA

    Plasmid

    #62717

    Purpose

    t1 gRNA (ATGAGAATCAAGGCGGTCGA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-SSFV-dCas9-GFP

    Plasmid

    #64105

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SSFV

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR1003

    Plasmid

    #71821

    Purpose

    Express Streptococcus pyogenes dCas9 (D10A, H840A)

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Neg. Ctrl_sgRNA

    Plasmid

    #111298

    Purpose

    CRISPR KO murine neg. ctrl.

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    NegCtrl sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEPOR0SP0013

    Plasmid

    #117521

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MsCLYBL_sgRNA1

    Plasmid

    #111294

    Purpose

    CRISPR KO murine CLYBL

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA1 against murine CLYBL
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgRNA AAVS1

    Plasmid

    #128119

    Purpose

    Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA AAVS1 + GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV/U6

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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