We narrowed to 13,568 results for: cas9 genes

1. pVH321-1-Tier1-PhCMV-dCas9-3xNLS

   Plasmid

   #169595

   Purpose

   Tier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

   Plasmid

   #161924

   Purpose

   To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting RDH11 exon 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJCC_162 SpCas9 KES(1107-1109)GG

   Plasmid

   #179526

   Purpose

   For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 KES(1107-1109)GG
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   replaced KES (residues 1107-1109) with GG

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

   Plasmid

   #176236

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176237

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176238

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eSpCas9(1.1)-ABL1-gRNA1-exon4

   Plasmid

   #163322

   Purpose

   eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ABL1 gRNA 1_Exon4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

8. eSpCas9(1.1)-ABL1-gRNA2-exon4

   Plasmid

   #163323

   Purpose

   eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ABL1 gRNA 2_Exon4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

   Plasmid

   #165083

   Purpose

   gRNA 2 of pair B for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

    Plasmid

    #165085

    Purpose

    gRNA 1 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

    Plasmid

    #165086

    Purpose

    gRNA 2 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-TREX1 g3 Cas9-T2A-mCherry

    Plasmid

    #164252

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUPD2 nickase D10A Cas9 (GB1691)

    Plasmid

    #160588

    Purpose

    Nickase D10A Cas9 for Nt fusion.

    Depositor

    Diego Orzaez

    Insert

    nickase D10A Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

    Plasmid

    #149346

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbc

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

    Plasmid

    #149347

    Purpose

    Mammalian expression vector for SpyCas9 and puromycin resistance

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

    Plasmid

    #159919

    Purpose

    Mutagenesis of Vamp1

    Depositor

    Larry Zweifel

    Insert

    Vamp1 (Vamp1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

    Plasmid

    #159907

    Purpose

    Mutagenesis of Netrin1

    Depositor

    Larry Zweifel

    Insert

    Netrin1 (Ntn1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-FLEX-SaCas9-U6-sgDcc

    Plasmid

    #159906

    Purpose

    Mutagenesis of Dcc

    Depositor

    Larry Zweifel

    Insert

    Dcc (Dcc Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid

    #132927

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Insert

    humanized SpCas9
    

    Use

    CRISPR and Synthetic Biology; Pmagic gateway entr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS

    Plasmid

    #121827

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-Cas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge