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pEJS1026-pMCSG7-HpaCas9

Plasmid

#121540

Purpose

Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

HpaCas9

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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SH3PXD2A-PX (1-126)

Plasmid

#119115

Purpose

Bacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SH3PXD2A-PX (1-126) (SH3PXD2A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1027-pMCSG7-SmuCas9

Plasmid

#121541

Purpose

Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

SmuCas9

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pY008 (pcDNA3.1-hPbCpf1)

Plasmid

#69980

Purpose

Expresses humanized LPbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
SNX11-PX (7-167)

Plasmid

#119092

Purpose

Bacterial expression of human phox homology (PX) domain, SNX11-PX (7-167)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX11-PX (7-167) (SNX11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-kan

Plasmid

#108706

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD6 Flag

Plasmid

#74898

Purpose

Express COMMD6 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD6 (COMMD6 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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SNX12-PX (1-162)

Plasmid

#119093

Purpose

Bacterial expression of human phox homology (PX) domain, SNX12-PX (1-162)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX12-PX (1-162) (SNX12 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic

Plasmid

#32736

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
UKNP.1.12.1

Plasmid

#97384

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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mCardinal-CytERM-N-17

Plasmid

#56158

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

CytERM

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
[pSK475] pLJC5 HA-Depdc5(A)

Plasmid

#109344

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant A

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

DIYGD(185-189)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK476] pLJC5 HA-Depdc5(B)

Plasmid

#109345

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant B

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

EQPLH(371-375)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK478] pLJC5 HA-Depdc5(P)

Plasmid

#109347

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant P

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

YDLLP(775-779)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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D5_Ld_280600

Plasmid

#135821

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

major surface protease gp63, putative,leishmanolysin, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pProEX-Af2Sir2

Plasmid

#8380

Depositor

Cynthia Wolberger

Article

Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Insert

Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)

Tags

His

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ARAF_S214C

Plasmid

#51077

Purpose

mammalian expression of ARAF S214C

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

S214C

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98976

Purpose

Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(N863A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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eGFP-pk-H302A

Plasmid

#115618

Purpose

Expresses human Parkin H302A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

H302A

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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mCardinal-MannII-N-10

Plasmid

#56167

Purpose

Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MannII

Tags

mCardinal

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEJS540-pCSDest2-AcrIIC4Hpa

Plasmid

#113434

Purpose

Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS542-pCSDest2-AcrIIC5Smu

Plasmid

#113435

Purpose

Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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PET11a-Af2sir2

Plasmid

#8383

Depositor

Cynthia Wolberger

Article

Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Insert

Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)

Expression

Bacterial

Mutation

PET11a-Af2Sir2D103N

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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mEos2-Alpha-Actinin-C-14

Plasmid

#57347

Purpose

Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-Talin-IBS2-7

Plasmid

#57424

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin IBS2 (Tln1 Mouse)

Tags

mEos2

Expression

Mammalian

Mutation

Talin Integrin Binding Site

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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SNX3-PX (1-162)

Plasmid

#119084

Purpose

Bacterial expression of human phox homology (PX) domain, SNX3-PX (1-162)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX3-PX (1-162) (SNX3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGL2 p15 4xSBR2 MutSBE

Plasmid

#15707

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR2 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutant SBE (Smad binding element)

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pFL His-SDS22

Plasmid

#113512

Purpose

Expression of His-SDS22 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

SDS22 (PPP1R7 Human)

Tags

6*His

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Psicheck2 MERTK full length 3'UTR

Plasmid

#26992

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

MERTK Full length 3' UTR (MERTK Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pLacI-GFP-HEC1 WT

Plasmid

#114049

Purpose

Expression of exogenous LacI-GFP-HEC1 WT

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

GFP-HEC1 (NDC80 Human)

Expression

Mammalian

Mutation

WT HEC1 sequence

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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