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pL0R_lacZ

Plasmid

#202095

Purpose

Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_o-1

Plasmid

#202091

Purpose

Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHPyV6-607a

Plasmid

#24727

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

Full genome of Human polyomavirus 6

Use

Viral clone

Available Since

June 8, 2010

Availability

Academic Institutions and Nonprofits only

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p3HM2Luc

Plasmid

#234807

Purpose

Mammalian expression of human muscarinic M2 receptor fused to RLuc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

Muscarinic M2 receptor

Tags

3xHA and RLuc8

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3HB2ALuc

Plasmid

#234808

Purpose

Mammalian expression of human B2-adrenergic receptor fused to RLuc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

B2-adrenergic receptor

Tags

3xHA and RLuc8

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-Septin 11 isoform II

Plasmid

#120317

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pKPY514

Plasmid

#62598

Purpose

Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr251Gly-EcPheRS

Tags

6xHis

Expression

Bacterial

Mutation

Thr251Gly

Promoter

T5

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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SEPT5 WT

Plasmid

#27272

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pBW842_pCAG-pExpr-FP-DECODER

Plasmid

#87551

Purpose

Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP, EGFP, iRFP720, mRuby2

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Myo 1b-myc

Plasmid

#135064

Purpose

Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

Depositor

Martin Bähler

Article

Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

Insert

Myo 1b with 5' UTR (Myo1b Rat)

Tags

Linker and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRS413-CUP1pr

Plasmid

#221109

Purpose

Copper-inducible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-luxKOk

Plasmid

#229944

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKOk

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-luxGold

Plasmid

#229943

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2-krt4-RFP

Plasmid

#97149

Purpose

As a control of miRNA sponge

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk3

Plasmid

#81012

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk3

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Muc2D1D2D3cysD1

Plasmid

#155214

Purpose

Expresses human MUC2 residues 21-1397

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3cysD1 (MUC2 Human)

Tags

6xHistadine

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3

Plasmid

#129257

Purpose

Express Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 xDvl-RFP

Plasmid

#29683

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

dishevelled (dvl2.L Frog)

Tags

RFP

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pCAG-GFPmG1

Plasmid

#50444

Purpose

Expression of a EGFP variant in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GFPmG1

Expression

Mammalian

Mutation

E143K, N147Q

Promoter

CAG

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pEIAV-SIN6.1 CBLacZ-LucWm1A

Plasmid

#44167

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

LacZ
firefly luciferase

Use

Lentiviral and Luciferase

Promoter

CB, CMV enhancer/chicken beta-actin promoter

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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