We narrowed to 63,044 results for: cloning

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pDONR223-ROR2

Plasmid

#23927

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ROR2 (ROR2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-SPHK1

Plasmid

#23704

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SPHK1 (SPHK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-HK2

Plasmid

#23854

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HK2 (HK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pENTRDsRedEx2

Plasmid

#22451

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

DsRedExpress

Use

Gateway Cloning

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag-HA-EIF3S5

Plasmid

#22546

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

EIF3S5 (EIF3F Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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BRCA1 (1-304)

Plasmid

#12645

Depositor

Rachel Klevit

Publication

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

E3-Ring domain, Ub-ligase (BRCA1 Human)

Tags

His

Expression

Bacterial

Available since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Publication

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6f

Plasmid

#207856

Purpose

Mammalian expression of PE6f prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6f

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-PE6g

Plasmid

#207857

Purpose

Mammalian expression of PE6g prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6g

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMV-Hylight-T152E

Plasmid

#193448

Purpose

A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)

Depositor

Richard Goodman

Publication

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Binding-Dead Hylight

Expression

Mammalian

Mutation

Threonine 67 (ACT) mutated to a Glutamic Acid (GA…

Promoter

CMV

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR_DKO

Plasmid

#183192

Purpose

Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

Depositor

Lang Li

Publication

Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6, mU6

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-EEA1

Plasmid

#176491

Purpose

For lentiviral transduction of 3FLAG-EEA1

Depositor

Wade Harper

Publication

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFLAG

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125693

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Publication

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pUCmini-iCAP-PHP.N

Plasmid

#127851

Purpose

non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.N VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA_RFP-TEVprotease

Plasmid

#124947

Purpose

Expression of RFP-TEVprotease

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-TEVprotease

Tags

mCherry

Expression

Mammalian

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-linker-M.EcoGII

Plasmid

#122082

Purpose

Express M.EcoGII with detabilization domain - inducible

Depositor

Laure Crabbe

Publication

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII

Use

Retroviral

Tags

V5 tag on Nter of M.EcoGII

Expression

Bacterial and Mammalian

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pScCas9

Plasmid

#117700

Purpose

Mammalian expression vector encoding WT ScCas9

Depositor

Joseph Jacobson

Publication

Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

Insert

Streptococcus canis Cas9

Use

CRISPR

Expression

Mammalian

Promoter

EF-1α

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-X1-hygro-mCherry-RAMP4

Plasmid

#118391

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EIF-1a promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

Plasmid

#102244

Purpose

Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

Depositor

Jacob Corn

Insert

dCas9-HA-BFP-KRAB-NLS

Use

CRISPR and Lentiviral

Tags

HA-TagBFP-KRAB-NLS

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMH-HA

Plasmid

#101767

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

Depositor

Michael Huen

Publication

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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