We narrowed to 7,296 results for: ef1a

1. pHR RPS27A-3xFlag_IRES-GFP

   Plasmid

   #198390

   Purpose

   RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FDIO-COX4-dAPEX2

   Plasmid

   #117178

   Purpose

   Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   COX4-dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-NES-mTagBFP2

   Plasmid

   #216129

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-MAPKKnucelarExportSignal-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HiFi dCas9 KRAB

   Plasmid

   #188500

   Purpose

   Expresses FLAG tagged HiFi dCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/R691A/H840A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP in pDBEcR-pIND(SP1)/Bla

   Plasmid

   #201557

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc and FLAG-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIMD(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FDIO-LACTB-dAPEX2

   Plasmid

   #117181

   Purpose

   Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   LACTB-dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX311 LacZ

   Plasmid

   #117731

   Purpose

   Open reading frame vector encoding LacZ

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   LacZ
   

   Use

   Lentiviral

   Tags

   2X V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PlmdCas12e KRAB

   Plasmid

   #188506

   Purpose

   Expresses FLAG tagged PlmdCas12e KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12e
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-FLAG-KRAB-NLS

   Expression

   Mammalian

   Mutation

   D659A/E756A/D922A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

   Plasmid

   #114376

   Purpose

   expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP, Kv2.1C, and TlcnC

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEBB-BT-Ubiquitin

    Plasmid

    #36098

    Depositor

    Ezra Burstein

    Article

    Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

    Insert

    Ubiquitin (RPS27A Human)
    

    Use

    Tags

    TB-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FDIO-SYP-HRP

    Plasmid

    #117187

    Purpose

    Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    SYP-HRP
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-eDIO-ChR2-EYFP

    Plasmid

    #104387

    Purpose

    PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

    Depositor

    Volker Busskamp

    Article

    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

    Insert

    Channelrhodopsin 2
    

    Use

    Cre/Lox; Piggybac

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E134R

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pONSY-Lifeact:mCherry

    Plasmid

    #111876

    Purpose

    This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Lifeact fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCh-466

    Plasmid

    #92251

    Purpose

    Plasmid for expression of GST-tagged human TNRC6C CED in mammalian cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690

    Promoter

    EF1A

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_103

    Plasmid

    #133460

    Purpose

    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE8e SpG Puro

    Plasmid

    #235044

    Purpose

    Adenosine base editor 8e* with SpG nicking Cas9 makes A > G edits

    Depositor

    Justin Pritchard

    Article

    Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

    Insert

    ABE8e, nCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-PuroR

    Expression

    Mutation

    Promoter

    EF1a core

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-EFS-MCP-3XBFP-NLS

    Plasmid

    #236372

    Purpose

    Overexpression of MCP-3XBFPnls in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    MCP-3XBFPnls
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EFS-mRuby2(miRE.FF4)-TS.FF6x1-bGH

    Plasmid

    #235309

    Purpose

    ComMAND open-loop circuit regulating mRuby2, EFS-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS (human EF1a short, intronless)

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF.DEST51-Dok1

    Plasmid

    #215458

    Purpose

    Expression vector with Dok1

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    DOK1 (DOK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. EFS-mRuby2(miRE.FF4)-TS.FF4x1-bGH

    Plasmid

    #235310

    Purpose

    ComMAND closed-loop circuit regulating mRuby2, EFS-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS (human EF1a short, intronless)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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