Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,750 results for: eng

Clear keywords
Showing: 2441 - 2460 of 16750 results

  1. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

    Enlarge

  4. pCMV-ABE8.20m-eVRQR-P2A-EGFP (LLH569)

    Plasmid
    #242656
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.20 A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.20m-VRQR-S55R-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA, eVRQR mutations in SpC…
    Promoter
    CMV and T7
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242656-plasmid-map-sequence-id-488970

    Enlarge

  5. pM-Cas9

    Plasmid
    #196325
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP
    Depositor
    Zhenghe Li
    Publication
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196325-plasmid-map-sequence-id-390015

    Enlarge

  9. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    91130-plasmid-map-sequence-id-182254

    Enlarge

  10. pDIRECT_25H

    Plasmid
    #91145
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91145-plasmid-map-sequence-id-182281

    Enlarge

  12. pAAV-mDlx-NLS-mRuby2 (AAV Retrograde)

    Viral prep
    #99130-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Publication
    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.
    Promoter
    mDLX5/6
    Tags
    mRuby2
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

    Plasmid
    #164514
    Purpose
    Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.
    Depositor
    Loren Looger
    Publication
    Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi
    Insert
    (Cyto)iGlucoSnFR-mRuby2
    Use
    AAV
    Mutation
    Synthetic Construct
    Promoter
    GFAP
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    164514-plasmid-map-sequence-id-324004

    Enlarge

  16. pAAV-syn-jYCaMP1s (AAV1)

    Viral prep
    #135424-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-syn-jYCaMP1s (#135424). In addition to the viral particles, you will also receive purified pAAV-syn-jYCaMP1s plasmid DNA. Synapsin-driven expression of calcium sensor YCaMP1s. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Kaspar Podgorski
    Publication
    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s
    Promoter
    Syn
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV_hSynapsin_psychLight2 (AAV9)

    Viral prep
    #163909-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV_hSynapsin_psychLight2 (#163909). In addition to the viral particles, you will also receive purified pAAV_hSynapsin_psychLight2 plasmid DNA. Synapsin-driven expression of the genetically encoded fluorescent sensor psychLight2 to detect behaviorally relevant serotonin release. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Publication
    Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003
    Promoter
    Syn
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2441 - 2460 of 16750 results