We narrowed to 6,485 results for: plasmid dna

1. BE3-YE1

   Plasmid

   #132943

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   BE3-(W90Y-R126E)
   

   Use

   CRISPR; Base editor

   Tags

   Expression

   Mammalian

   Mutation

   BE3-(W90Y-R126E)

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p350_LTJ_180bpCD90.1Template

   Plasmid

   #104988

   Purpose

   180bp HDR template for murine CD90.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   180bp genomic DNA sequence from murine CD90.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC28-hKdm2b-CxxC-PHD

   Plasmid

   #49216

   Purpose

   Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.

   Depositor

   Rob Klose

   Insert

   Kdm2b (KDM2B Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. p214_LTJ_1kbCD90.1Template

   Plasmid

   #82667

   Purpose

   1kb HDR template for murine CD90.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   1kb genomic DNA sequence from murine CD90.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p229_LTJ_2kbCD90.1Template

   Plasmid

   #82668

   Purpose

   2kb HDR template for murine CD90.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   2kb genomic DNA sequence from murine CD90.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p248_LTJ_2kbCD45.1Template

   Plasmid

   #82662

   Purpose

   2kb HDR template for murine CD45.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   2kb genomic DNA sequence from murine CD45.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p213_LTJ_160bpCD90.1Template

   Plasmid

   #82666

   Purpose

   160bp HDR template for murine CD90.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   160bp genomic DNA sequence from murine CD90.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p230_LTJ_4kbCD90.1Template

   Plasmid

   #82669

   Purpose

   4kb HDR template for murine CD90.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   4kb genomic DNA sequence from murine CD90.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p242_LTJ_1kbCD45.1Template

   Plasmid

   #82661

   Purpose

   1kb HDR template for murine CD45.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   1kb genomic DNA sequence from murine CD45.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DDI1

    Plasmid

    #32867

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DDI1 (DDI1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids 239-367 only

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pChd4EosHaloTAF_Puro

    Plasmid

    #172099

    Purpose

    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion

    Depositor

    Brian Hendrich

    Insert

    chromodomain helicase DNA binding protein 4 (Chd4 Synthetic, Mouse)
    

    Use

    Mouse Targeting

    Tags

    3xFLAG, Avi, Eos, Halo, and TEV

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p243_LTJ_4kbCD45.1Template

    Plasmid

    #82663

    Purpose

    4kb HDR template for murine CD45.1

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    4kb genomic DNA sequence from murine CD45.1
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Human SLC overexpression library

    Pooled Library

    #213694

    Purpose

    Inducible barcoded library with 467 codon-optimized cDNAs with UMIs to test the impact of SLC transporters on biological processes.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

14. pSANG10-3F-BG4

    Plasmid

    #55756

    Purpose

    Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

    Depositor

    Shankar Balasubramanian

    Article

    Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

    Insert

    BG4
    

    Use

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCap-101-Apr

    Plasmid

    #227507

    Purpose

    Capture plasmid with apramycin resistance and pIJ101 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Bxb1-GT_AIO-TetOn_Divergent Donor

    Plasmid

    #229784

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Bxb1-GA_AIO-TetOn_Divergent Donor

    Plasmid

    #229786

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge