We narrowed to 7,305 results for: plasmid dna

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pAAV-rBrokenheart

Plasmid

#203394

Purpose

AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

Depositor

Joseph Dougherty, Rob Mitra

Article

Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

Insert

Brokenheart

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_GW_Dest

Plasmid

#86955

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

Depositor

Joseph Dougherty

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pICE-HA-CtIP-siR-N289A-H290A

Plasmid

#82032

Purpose

Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Tags

HA

Expression

Mammalian

Mutation

Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

Plasmid

#200634

Purpose

Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

PTEN (PTEN Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pICE-HA-CtIP-siR-S664A-S679A-S745A

Plasmid

#82031

Purpose

Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Tags

HA

Expression

Mammalian

Mutation

Non phosphorylatable mutations on 3 of the main A…

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGMC00018 (aka pRC0082)

Plasmid

#195320

Purpose

Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

Depositor

Raj Chari

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Mammalian

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSc1-DD

Plasmid

#80439

Purpose

Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

Depositor

Ervin Welker

Article

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP

Use

CRISPR

Tags

E. coli dihydrofolate reductase

Expression

Mammalian

Promoter

CMV and U6

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSc1-puro

Plasmid

#80438

Purpose

Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

Depositor

Ervin Welker

Article

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP
Puromycin resistance

Use

CRISPR

Expression

Mammalian

Promoter

CMV, U6, and hPGK

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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EGFR(L858R,T790M)-NanoLuc Fusion Vector

Plasmid

#237199

Purpose

Express EGFR(L858R,T790M)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

EGFR (EGFR Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

L858R/T790M double mutations

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-SMARCA2 Fusion Vector

Plasmid

#238641

Purpose

Express NanoLuc-SMARCA2 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

SMARCA2 (SMARCA2 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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AKT3-NanoLuc Fusion Vector

Plasmid

#237208

Purpose

Express AKT3-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

AKT3 (AKT3 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SMARCA4-NanoLuc Fusion Vector

Plasmid

#238638

Purpose

Express SMARCA4-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

SMARCA4 (SMARCA4 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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PD-L1-NanoLuc Fusion Vector

Plasmid

#237129

Purpose

Express PD-L1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

PD-L1 (CD274 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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CBP (bd1)-NanoLuc Fusion Vector

Plasmid

#236945

Purpose

Express CBP (bd1)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

CBP (CREBBP Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

Bromodomain 1

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-SMARCA4 Fusion Vector

Plasmid

#238639

Purpose

Express NanoLuc-SMARCA4 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

SMARCA4 (SMARCA4 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-TAF1 Fusion Vector

Plasmid

#237165

Purpose

Express NanoLuc(R)-TAF1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

TAF1 (TAF1 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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hCHMP4B-NanoLuc Vector

Plasmid

#236973

Purpose

Express hCHMP4B-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

hCHMP4B (CHMP4B Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-hCHMP4B Vector

Plasmid

#236974

Purpose

Express NanoLuc(R)-hCHMP4B Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

hCHMP4B (CHMP4B Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGL4 [luc2P/RBP-Jk-RE/Hygro] Vector

Plasmid

#236804

Purpose

Express RBP-Jk response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

RBP-Jk (RBPJ Human)

Use

Luciferase

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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JARID1BA-c539

Plasmid

#79643

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

JARID1B (KDM5B Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Promoter

polyhedrin

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only