We narrowed to 2,492 results for: PUC19
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Plasmid#184373PurposeMammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editorDepositorInsertdABE-SuperFi-Cas9
UseCRISPRTagsFLAG, SV40 NLS, and nucleoplasmin NLSExpressionMammalianMutationD10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…PromoterEF-1α core promoterAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15545_dABE8e-SuperFi-Cas9
Plasmid#184375PurposeMammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editorDepositorInsertdABE8e-SuperFi-Cas9
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationD10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…PromoterEF-1α core promoterAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ir7f-T2A-QF2 HDR plasmid
Plasmid#140942PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f geneDepositorInsertIr7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
Mutation4 point mutations (annotated on plasmid map) were…Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF445-donor
Plasmid#112332PurposeCRISPR donor plasmid to tag human transcription factor ZNF445 with GFPDepositorInsertZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
UseCRISPRMutationrs78585428, hg38:3:44446218:C>T, hg38:3:444466…Available SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
23SrRNA.RAV12(wt).2508-2580.G2530AAAC
Plasmid#26190DepositorInsert23S ribosomal RNA fragment (modified)
ExpressionBacterialMutationfragment 2508-2580 of E. coli rRNA, with nucleoti…Available SinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGGI_Myr-YFP-MiniTurboID
Plasmid#222432PurposePotential plasma membrane control.DepositorInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker, Myr, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV12 [psng-1::SNG-1::CRY2olig(535)]
Plasmid#197596PurposePan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegansDepositorExpressionWormMutationE490GAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U
Plasmid#26191DepositorInsert23S ribosomal RNA fragment (modified) with G2553U mutation
ExpressionBacterialMutationfragment 2508-2580 of E. coli rRNA, with nucleoti…Available SinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
23SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH90
Plasmid#26192DepositorInsert23S ribosomal RNA fragment (modified) with duplex H90
ExpressionBacterialMutationfragment 2508-2580 of E. coli rRNA, with nucleoti…Available SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]
Plasmid#197599PurposePan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactiveDepositorExpressionWormMutationD387A, E490GAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC004 - PFS library in Beta-lactamase gene Target Plasmid
Pooled Library#79153PurposeLibrary of protospacer flanking sites (PFS) inserted at the 5' end of the β-lactamase gene for screening PFS preference for Cas13a/C2c2.DepositorAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT1219
Plasmid#228301Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variantDepositorInsertMFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Synthetic, Severe acute respiratory syndrome-related coronavirus 2)
UseSynthetic BiologyTagsGGG linker followed by His6 and MF_ secretion sig…ExpressionYeastMutationG339D, S371L, S373P, S375F, K417N, N440K, G446S, …Promoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
35S:5mARF17 pLBR19
Plasmid#12073DepositorInsert5mARF17 (ARF17 Mustard Weed)
ExpressionPlantMutationmiR160-resistant version of ARF17 has five silent…Available SinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTPT
Plasmid#1962DepositorInsertTPT
ExpressionBacterialMutation2 copies of 20bp artificial nucleosome positionin…Available SinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
plIG-109_CD19-BBz_CAR_SR_Library
Pooled Library#207476PurposeCD19-BBz CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cellsDepositorUseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-123_HA-GD2-28z_CAR_TF_Library
Pooled Library#207478PurposeHA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into thDepositorSpeciesHomo sapiensUseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-128_HA-GD2-28z_CAR_TFxTF_Library
Pooled Library#207480PurposeHA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular pooleDepositorUseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Spike (S) Ectodomain Library C
Pooled Library#157973PurposeEncodes Spike ectodomain mutations that stabilize 'up' prefusion conformationDepositorExpressionBacterial and YeastSpeciesSars-cov-2Available SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Spike (S) Ectodomain Library B
Pooled Library#157972PurposeEncodes Spike ectodomain mutations that stabilize 'up' prefusion conformationDepositorExpressionBacterial and YeastSpeciesSars-cov-2Available SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Spike (S) Ectodomain Library A
Pooled Library#157971PurposeEncodes Spike ectodomain mutations that stabilize 'up' prefusion conformationDepositorExpressionBacterial and YeastSpeciesSars-cov-2Available SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only