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GTU-g

Plasmid

#81094

Purpose

Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

sfGFP

Use

Cre/Lox

Tags

sfGFP

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2-V660N

Plasmid

#12292

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y and V660N; signal peptide (aa1-23) re…

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmNot10

Plasmid

#79254

Purpose

Expresses EGFP-tagged DmNot10 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot10 (Not10 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot11

Plasmid

#79253

Purpose

Expresses EGFP-tagged DmNot11 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot11 (Not11 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-HMGB1

Plasmid

#183198

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-HMGB1

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-RFC4

Plasmid

#183199

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC4

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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NOVA1_Full-length_pGEX

Plasmid

#135147

Purpose

Recombinant expression of NOVA1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

NOVA1 (NOVA1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

Plasmid

#83286

Purpose

untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

mTurquoise2-FHA2-AURKB substrate-YPet

Use

Retroviral

Expression

Mammalian

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE660

Plasmid

#153258

Purpose

M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Expression

Insect

Mutation

P418S

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm4_A

Plasmid

#145883

Purpose

Insect Expression of DmLSm4

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm4 (LSm-4 Fly)

Tags

V5-6xHis

Expression

Insect

Mutation

two silent mutations compared to the sequence of …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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PCBP4_3xKH_pGEX

Plasmid

#135156

Purpose

Recombinant expression of PCBP4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PCBP4 (PCBP4 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

Plasmid

#61939

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-156)

Tags

6x HIS

Expression

Bacterial

Mutation

Construct ends at residue 156

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST

Plasmid

#175017

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

OK-HEFwtED

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND2_L17_S19_S11

Plasmid

#132730

Purpose

T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND2_L17_S19_S11

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40

Plasmid

#79251

Purpose

Expresses EGFP-tagged DmCaf40 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCaf40 (Rcd-1 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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RBMS3_2xRRM_pGEX

Plasmid

#135142

Purpose

Recombinant expression of RBMS3 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBMS3 (RBMS3 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

July 10, 2021

Availability

Academic Institutions and Nonprofits only

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RBM24_RRM_pGEX

Plasmid

#135148

Purpose

Recombinant expression of RBM24 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM24 (RBM24 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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