We narrowed to 9,647 results for: virus

1. rAAV2-retro helper

   Plasmid

   #81070

   Purpose

   retrograde AAV helper vector, carrying rep and modified capsid gene

   Depositor

   David Schaffer, Alla Karpova

   Article

   Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

   Inserts

   rep
   rAAV2-retro cap
   

   Use

   AAV and Unspecified; Helper vector

   Tags

   Expression

   Mutation

   Pro2Ala, possibly Asp613Gly and changes made to A…

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Ikkb-FLAG(K44A)

   Plasmid

   #23299

   Depositor

   Warner Greene

   Article

   Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

   Insert

   Ikkbeta (IKBKB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

3. MSCV-opEBNA2-IRES-GFP

   Plasmid

   #220354

   Purpose

   Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   3 x HA tag

   Expression

   Mammalian

   Mutation

   codon-optimised for expression in mammalian cells

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMP71-HLA-A0201-His

   Plasmid

   #108214

   Purpose

   HLA-A0201 his tagged

   Depositor

   Sébastien Walchli

   Article

   Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

   Insert

   major histocompatibility complex, class I, B (HLA-A Human)
   

   Use

   Retroviral

   Tags

   his

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26

   Plasmid

   #199262

   Purpose

   Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

   Insert

   NmeABE8e
   

   Use

   AAV and CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189923

   Purpose

   AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e V106W
   

   Use

   AAV

   Tags

   Expression

   Mutation

   nSaCas9 KKH, TadA ABE8e V106W

   Promoter

   EFS

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-P301L Tau

   Plasmid

   #176267

   Purpose

   This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

   Depositor

   Tsuneya Ikezu

   Article

   You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

   Has Service

   AAV1

   Insert

   Flex-P301L tau (MAPT )
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-MMLV-gag-hMLH1dn

   Plasmid

   #195513

   Purpose

   MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn (MLH1 Human)
   

   Use

   Virus like particle

   Tags

   FLAG, NES, and Protease cleavate site

   Expression

   Mammalian

   Mutation

   Human MLH1 del754-756

   Promoter

   CMV

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-C1 actin-3XNLS P2A mCherry

   Plasmid

   #58475

   Purpose

   Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Myc-AIM2

    Plasmid

    #73958

    Purpose

    Express c-Myc-tagged human AIM2 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

    Insert

    absent in melanoma 2 (AIM2) (AIM2 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189924

    Purpose

    AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e V106W
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SaCas9 D10A, TadA ABE8e V106W

    Promoter

    EFS

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-C-prM-E-NS1

    Plasmid

    #175281

    Purpose

    lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    C-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
    

    Use

    Lentiviral

    Tags

    IRES EGFP

    Expression

    Mutation

    Promoter

    human ubiquitin C

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF160_VSV-G

    Plasmid

    #225963

    Purpose

    CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-VSVG (env protein)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cbh_v5 AAV-saCBE N-terminal

    Plasmid

    #137182

    Purpose

    AAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-saCBE N-terminal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-Syn-NS1

    Plasmid

    #175276

    Purpose

    AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    NLS-dTomato (P2A cleavage)

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cbh_v5 AAV-saCBE_KKH C-terminal

    Plasmid

    #137184

    Purpose

    AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE, and U6-sgRNA (KKH variant).

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-saCBE_KKH C-terminal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E782K;N968K;R1015H conferring recognition of NNNR…

    Promoter

    Cbh

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. NSP4-WT-Flag

    Plasmid

    #210339

    Purpose

    Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid

    #118586

    Purpose

    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

    Depositor

    Radhakrishnan Padmanabhan

    Article

    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

    Insert

    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    

    Use

    Yeast-e. col shuttle vector prs424

    Tags

    Expression

    Mutation

    The conserved GDD in the NS5 polymerase gene muta…

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGT9569

    Plasmid

    #219431

    Purpose

    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Tags

    PapE-4LF2

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-TRE-NS1NF

    Plasmid

    #175279

    Purpose

    AAV vector mediating inducible expression of the NS1 gene of YFV-17D

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    NS1 (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tight TRE

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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