We narrowed to 2,956 results for: NAK

1. pKI-cjACTB-EGFP-5'short

   Plasmid

   #129748

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   ACTB (ACTB C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKI-cjACTB-EGFP

   Plasmid

   #129747

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   ACTB (ACTB C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIR-192-Reporter

   Plasmid

   #71872

   Purpose

   Mammalian expression vector for the analysis of miR-19α activity

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   Reverse complementary sequence of miR-192
   

   Use

   Tags

   firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pMIR-193a-5p-Reporter

   Plasmid

   #71873

   Purpose

   Mammalian expression vector for the analysis of miR-193a-5p activity

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   Reverse complementary sequence of miR-193a-5p
   

   Use

   Tags

   firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pMIR-31-192-193a-Reporter

   Plasmid

   #71874

   Purpose

   Mammalian expression vector for the analysis of miR-31-19α-193a activity

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   Binding sequence targeted by miR-31, miR192, and miR-193a-5p
   

   Use

   Tags

   firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

6. RGS-6xHis-LIN-29-pcDNA3.1-

   Plasmid

   #52515

   Purpose

   expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Lin-29 b (lin-29 Nematode)
   

   Use

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   wt (changed coding sequence in 5' (5th codon…

   Promoter

   CMV

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pClneoMyc-LATS2

   Plasmid

   #66852

   Purpose

   Express Myc-tagged LATS2 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

   Insert

   LATS2 (LATS2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLAG-YAP1

   Plasmid

   #66853

   Purpose

   Express FLAG-tagged YAP1 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV enAsCas12f-HKRA-sgRNA_v7

   Plasmid

   #208357

   Purpose

   Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-HKRA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I123H/D195K/D208R/V232A

   Promoter

   CMV

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330 p53

    Plasmid

    #59910

    Purpose

    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting p53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid

    #107270

    Purpose

    Expresses Cas9 upon induction with doxicycline.

    Depositor

    Pablo Menéndez

    Article

    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

    Inserts

    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    

    Use

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid

    #202824

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 - two

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85008

    Purpose

    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMMP-MCS-IRES-eGFP-P2A-iCas9

    Plasmid

    #178533

    Purpose

    For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

    Depositor

    Ryoji Yao

    Article

    Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

    Insert

    iCaspase9 (CASP9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85006

    Purpose

    For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330 Pten

    Plasmid

    #59909

    Purpose

    pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Pten (Pten Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLEX307-mFzd7

    Plasmid

    #102869

    Purpose

    Expresses mouse Fzd7 in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Frizzled 7 (Fzd7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-Tight-Puro-KrasG12D2B

    Plasmid

    #139786

    Purpose

    Lentiviral tet-inducible expression of KrasG12D (mouse)

    Depositor

    Joan Massague

    Article

    Su et al Nature. 2020 Jan;577(7791):566-571. doi:

    Insert

    KrasG12D (Kras Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pnEA-vH_His-TEV-SEPT2_SEPT6

    Plasmid

    #174497

    Purpose

    bacterial co-expression of human SEPT2 and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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