We narrowed to 24,405 results for: nes

1. pMSR:MSMEG_0301:Dendra

   Plasmid

   #159050

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0301
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSR:MSMEG_0225:Dendra

   Plasmid

   #159043

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0225
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSR:MSMEG_0710:Dendra

   Plasmid

   #159020

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0710
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSR:MSMEG_0384:Dendra

   Plasmid

   #159013

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0384
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSR:MSMEG_0229:Dendra

   Plasmid

   #159005

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0229
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSR:MSMEG_0024:Dendra

   Plasmid

   #158997

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0024
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AF10_OM-LZ

   Plasmid

   #111753

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Article

   Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

   Insert

   MLLT10 (MLLT10 Human)
   

   Use

   Tags

   6x His - TEV

   Expression

   Bacterial

   Mutation

   Contains the OM-LZ region of AF10 (residues 720-7…

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 3` CC: Hygro – GFP-N – loxP – mScarlet

   Plasmid

   #219564

   Purpose

   3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   3` CC: Hygro – GFP-N – loxP – mScarlet
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EF1-a

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5` CC: Puro – loxP – GFP-C

   Plasmid

   #219563

   Purpose

   5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   hPGK-promoter_PuroR_2A_loxP_GFP-C
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLXC2B-PLAG1-3xFLAG

    Plasmid

    #228057

    Purpose

    lentiviral expression of human PLAG1 with 3xFLAG tag

    Depositor

    Kenneth Chen, James Amatruda

    Article

    Chen et al Genes Dev. 2018 Aug 1;32(15-16):996-1007

    Insert

    PLAG1-3xFLAG (PLAG1 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX037

    Plasmid

    #167156

    Purpose

    MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    caffeic acid cycle genes
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pNOS, p35s

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. KAT5 sgRNA1

    Plasmid

    #138187

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT5

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT5 (KAT5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. KAT5 sgRNA2

    Plasmid

    #138188

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT5

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT5 (KAT5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV phSyn1(S)-FLEX-EGFP-WPRE

    Plasmid

    #51504

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    phSyn1

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV phSyn1(S)-FLEX-tdTomato-WPRE

    Plasmid

    #51505

    Purpose

    Can be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    phSyn1

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cas9/pTREX-n

    Plasmid

    #68708

    Purpose

    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    

    Use

    CRISPR

    Tags

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

    Expression

    Mutation

    C4239T mutation that eliminates a BamHI restricti…

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid

    #122853

    Purpose

    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    Cas9-GFP
    U6-sgUPRT
    

    Use

    CRISPR; Cas9-gfp plasmid for genome editing of cr…

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Cryptosporidium parvum U6 and Cryptosporidium par…

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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