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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hKif5b(1-560)rigor-2xmNeongreen

Plasmid

#174649

Purpose

Live-cell marker for stable microtubules

Depositor

Lukas Kapitein

Article

Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

Insert

hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)

Tags

mNeongreen-mNeongreen

Expression

Mammalian

Mutation

rigor mutation G234A

Promoter

Chicken beta-actin

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

Plasmid

#231986

Purpose

Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

OVA-2A-ZsGreen

Use

Lentiviral

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Nanog

Plasmid

#28221

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

Insert

Nanog (NANOG Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2011

Availability

Academic Institutions and Nonprofits only

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pCXLE-hGLIS1

Plasmid

#37625

Purpose

Non-integrating (episomal) expression of human GLIS1

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

human GLIS1 (GLIS1 Human)

Expression

Mammalian

Mutation

T110A, A187G, I221T

Promoter

CAG

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Lhx2

Plasmid

#61537

Purpose

Expresses Lhx2 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Lhx2 (Lhx2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
TetO-FUW-PLZF

Plasmid

#61543

Purpose

Expresses PLZF in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

PLZF (ZBTB16 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

I380T and G580D

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiDP1.0

Plasmid

#171094

Purpose

lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2

Depositor

Dario Palmieri

Article

Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

Insert

OsTIR1-mEmerald-P2A-miniAID-mCherry2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLOVE-Nmyc

Plasmid

#15951

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Available Since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only
Flag‐hTim1

Plasmid

#49207

Purpose

expresses Flag-tagged human Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (HAVCR1 Human)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
pFUW-TetO-ZFP36

Plasmid

#178438

Purpose

doxycycline-inducible expression of Human ZFP36 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

ZFP36 (ZFP36 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcdna DSG-2 TS

Plasmid

#89465

Purpose

DSG-2 tension sensor, using TSmod inserted in human DSG-2

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Expression

Mammalian

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Baf60c

Plasmid

#72691

Purpose

Doxycyline regulated expression of Baf60c in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Brg 1 (SMARCD3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-ORAI1-EC-HA

Plasmid

#174335

Purpose

encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Tags

GFP in the N-terminus and HA tag in the extracell…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Esrrb

Plasmid

#40798

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Esrrb (Esrrb Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pSAM2_mCherry_Tbx20

Plasmid

#72697

Purpose

Doxycyline regulated expression of Tbx20 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Tbx20 (Tbx20 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Ctcf

Plasmid

#40801

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Ctcf (Ctcf Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Junb

Plasmid

#178441

Purpose

doxycycline-inducible expression of Mouse Junb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Junb (Junb Mouse)

Use

Lentiviral

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC13-hygro

Plasmid

#231988

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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