We narrowed to 11,615 results for: VARS

1. pTW195b63

   Plasmid

   #136931

   Purpose

   Directed evolution of SpCas11

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gIII(N)-npuN
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCLX-UBI-mCherry

   Plasmid

   #114275

   Purpose

   Ubiquitous expression of the mCherry living color

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSETB-de4GFP

   Plasmid

   #85472

   Purpose

   Inducible expression of dual emitting pH sensitive GFP in bacterial cells

   Depositor

   S. James Remington

   Article

   Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

   Insert

   de4GFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S65T/C48S/H148C/T203C

   Promoter

   T7

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ211_WT_low

   Plasmid

   #128848

   Purpose

   Low copy number plasmid for expression of E. coli flagellin from its natural promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Expression

   Bacterial

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA_e-2

   Plasmid

   #202097

   Purpose

   Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. mTFP1-Lifeact-7

   Plasmid

   #54749

   Purpose

   Localization: Actin, Excitation: 462, Emission: 492

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Tags

   mTFP1

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

7. Lenti-EF1alpha-scFv-VP64_Blast

   Plasmid

   #192655

   Purpose

   3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   scFv-VP64
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJYDNgp

   Plasmid

   #162453

   Purpose

   Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-2Glinker-eUnaG2 (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   HA nad myc and eUnaG2 fluorescent protein

   Promoter

   GAL1

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1.1

   Plasmid

   #162737

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSADdeltaG-ChR2-mCherry

    Plasmid

    #32646

    Purpose

    Rabies vector pSADΔG expressing ChR2-mCherry for optogenetic excitation

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    ChR2
    

    Use

    Rabies virus

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKSB-609 U6-sgRNA1 ATCC GGAA

    Plasmid

    #173671

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZH514

    Plasmid

    #102667

    Purpose

    Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX165-Sniper-Cas9

    Plasmid

    #140560

    Purpose

    Expresses Sniper Cas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Mutation

    F539S M763I K890N

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a_speG_Cterm_His

    Plasmid

    #220483

    Purpose

    heterologous expression for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    speG
    

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZH516

    Plasmid

    #102669

    Purpose

    Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKSB-610 U6-sgRNA2 GGAA AGAG

    Plasmid

    #173672

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX165-Cas9-HF1

    Plasmid

    #140565

    Purpose

    Expresses Cas9-HF1 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Mutation

    N497A R661A Q695A Q926A

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a_PA4114_Cterm_His

    Plasmid

    #220484

    Purpose

    heterologous expression of PA4114 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA4114
    

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD/HisD-TagRFP675

    Plasmid

    #44274

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Expression

    Bacterial

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    pBAD

    Available Since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJY3919

    Plasmid

    #113332

    Purpose

    Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Expression

    Yeast

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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