We narrowed to 144,691 results for: ins

1. pIVT-EGFP-mouseCENP-C

   Plasmid

   #174444

   Purpose

   in vitro transcription template for mouse CENP-C (N-ter EGFP tagged)

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Centromere protein C
   

   Use

   In vitro transcription template

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   T7 SP6

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. POM43-mCherry

   Plasmid

   #69857

   Purpose

   N-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mCherry minus start and stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPI420

   Plasmid

   #113230

   Purpose

   safe haven knock-in plasmid for the act5 locus, Expression of H2B-mCherry

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   H2B
   

   Use

   Safe haven knock-in plasmid for the act5 locus, h…

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   act5

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD8A-iRGR-r11

   Plasmid

   #74369

   Purpose

   This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   RGR-r11
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AHD1

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. p6607 MSCV-IP N-HAonly PTPN18

   Plasmid

   #35007

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   PTPN18 (PTPN18 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   Feb. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pAB035a

   Plasmid

   #79190

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Plac62 gIII, luxAB; Ptet rpoZ-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac62; Ptet

   Available since

   Dec. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. POM42-mCitrine

   Plasmid

   #69854

   Purpose

   N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mCitrine minus start and stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HOXA9 (murine) HIS-tag pET

   Plasmid

   #8492

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HoxA9 (Hoxa9 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

9. Desmocollin2_tail-GST

   Plasmid

   #36992

   Depositor

   Kathleen Green

   Article

   Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

   Insert

   Dsc2 (DSC2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Intracellular domain of Dsc2 (nt 2,603–3,158)

   Promoter

   Available since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pJW4.21

    Plasmid

    #62875

    Purpose

    Bacterial expression of MBP-MEG-1

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    MBP-MEG-1
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFAST-BAC-Cdc20wt (2193)

    Plasmid

    #39850

    Depositor

    Jonathon Pines

    Article

    Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

    Insert

    Cdc20 (CDC20 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

12. POM43-mCitrine

    Plasmid

    #69855

    Purpose

    N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCitrine minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS YBX1

    Plasmid

    #19217

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    YBX1 (YBX1 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

14. PI(LM)-Rac1(WT)dc1.FLARE

    Plasmid

    #87590

    Purpose

    PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    PBD
    Rac1
    

    Use

    Tags

    YPet and dTurq

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b-At 14-3-3 phi

    Plasmid

    #44939

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 phi (GF14 PHI Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pAB029f

    Plasmid

    #79220

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ lambda cI-SH2ABL1; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ; PlacIq

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRPB1-CTD14

    Plasmid

    #112822

    Purpose

    pSMF2 with 14 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAB031a

    Plasmid

    #79221

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ HA4-Zif268; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ; PlacIq

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15b-At 14-3-3 lambda

    Plasmid

    #44942

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 lambda (GRF6 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET28-his-3C_Clu3B6p

    Plasmid

    #100093

    Purpose

    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

    Insert

    14-3-3 sigma human (SFN Human)
    

    Use

    Tags

    HSPB6 peptide and His6

    Expression

    Bacterial

    Mutation

    75-77 residues changed to AAA to facilitate cryst…

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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