We narrowed to 19,532 results for: iNOS

1. pCAG-lox-inv[Talpha1-HA-Dre]-lox-Akna-mOrange2

   Plasmid

   #196893

   Purpose

   Neuron-specific expression of Akna fused to mOrange2. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Akna-mOrange2

   Plasmid

   #196880

   Purpose

   Neuron-specific expression of the centrosomal protein Akna fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9

   Plasmid

   #196897

   Purpose

   Neuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   2xHA-Akap9(128-425) (Akap9 Rat)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

   Plasmid

   #196894

   Purpose

   Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   N-gTBD-mOrange2 (NEDD1 Human)
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3

   Plasmid

   #196895

   Purpose

   Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   2xHA-CAMSAP3 (Camsap3 Mouse)
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBactin-mOrange2-Cdk5rap2-CTD

   Plasmid

   #196855

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

   Plasmid

   #196856

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lacking the centrosome-targeting domain (CTD)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-inducible 3xFlag Mouse Caspase-9 ΔLS

   Plasmid

   #194609

   Purpose

   Inducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit Domain

   Depositor

   Hannah Rabinowich

   Article

   Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

   Insert

   N-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Val 177 to Asp 353 (Casp9 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Deletion of Large Subunit Domain eliminates bindi…

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD

   Plasmid

   #194608

   Purpose

   Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus CARD Domain

   Depositor

   Hannah Rabinowich

   Article

   Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

   Insert

   N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176 (Casp9 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Deletion of N-terminal CARD domain and changed Cy…

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-inducible 3xFlag Mouse Caspase-9 ΔB LS

    Plasmid

    #194927

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit Region Ala 264 to Asp 353

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Ala 264 to Asp 353 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Deletion of Large Subunit Region Ala 264 to Asp 3…

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUG36-NES-EGFP-cPHx3

    Plasmid

    #183668

    Purpose

    PtdIns(3,4)P2 biosensor for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Human, Frog)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Yeast

    Mutation

    Amino acids 169-329 (tandem trimer)

    Promoter

    MET25

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUG34-NES-EGFP-cPHx3

    Plasmid

    #183669

    Purpose

    PtdIns(3,4)P2 biosensor for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Human, Frog)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Yeast

    Mutation

    Amino acids 169-329 (tandem trimer)

    Promoter

    MET25

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. NES-mCherry-PH-BTKx1

    Plasmid

    #183653

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Human, Frog)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-mCherry

    Expression

    Mammalian

    Mutation

    Amino acids 2-170

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid

    #107942

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R, and N282A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGGG-Sr43

    Plasmid

    #186974

    Purpose

    Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

    Depositor

    Brande Wulff

    Article

    Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

    Insert

    Sr43
    

    Use

    Tags

    None

    Expression

    Bacterial and Plant

    Mutation

    None

    Promoter

    Natice

    Available Since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOmnibac_ZZ_linker_ybbR_Tau MTBD

    Plasmid

    #159719

    Purpose

    Expresses microtubule-binding domain of tau (amino acids 242-367) in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    Tau microtubule-binding domain only (amino acids 242-367) (MAPT Human)
    

    Use

    Tags

    ZZ affinity tag, ybbR tag

    Expression

    Insect

    Mutation

    Only amino acids 242-367

    Promoter

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-GGA3-VHS-GAT

    Plasmid

    #173719

    Purpose

    VHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.

    Depositor

    Juan Bonifacino

    Article

    Dell'Angelica et al J Cell Biol. 2000 Apr 3;149(1):81-94. do

    Insert

    GGA3 (GGA3, GGA2, GGA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Myc-gamma2/deltaILTM4

    Plasmid

    #119735

    Purpose

    GABAA receptor expression (chimeric rat rat gamma 2 subunit whose large intracellular loop, TM4 and C-term have been replaced with the equivalent domains of the rat delta subunit) C-myc near N-term

    Depositor

    Angel de Blas

    Article

    Wu et al J Biol Chem. 2012 Aug 10;287(33):27417-3

    Insert

    GABRG2/GABRD (Gabrg2, Gabrd Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]

    Plasmid

    #196887

    Purpose

    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔA LS

    Plasmid

    #194926

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Large Subunit Region Val 177 to Arg 263

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Val 177 to Arg 263 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to Eliminate Prot…

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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