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NAX_CAGGS_CAS9_m4_KRAB_mcherry

Plasmid

#167878

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pCS2-sod1

Plasmid

#107594

Purpose

Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod1 (sod1 Zebrafish)

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-41

Plasmid

#172757

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-ZFD_R

Plasmid

#198277

Purpose

For loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFD_R

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-42

Plasmid

#172758

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-42

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-10

Plasmid

#172743

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-10

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.T7 NFAT3

Plasmid

#28223

Depositor

Sebastien Jauliac

Article

Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

Insert

human NFAT3 (NFATC4 Human)

Tags

T7

Expression

Mammalian

Mutation

G160A

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pLenti-hSyn-Gβγ-BERKY1

Plasmid

#158429

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-GRK3ct

Use

Lentiviral

Expression

Mammalian

Promoter

hSynapsin

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMuLE ENTR SV40 PuroR L3-L2

Plasmid

#62164

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Puromycin

Use

Gateway Cloning

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-LOV

Plasmid

#22033

Purpose

Mammalian expression of mVenus tagged LOV2 domain

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-LOV

Use

Retroviral

Expression

Mammalian

Mutation

Insert length based on Venus (720bp) + LOV (450bp)

Available Since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
pHis-zfEpoa

Plasmid

#65611

Purpose

Express soluble His-tagged zebrafish erythropoietin in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

Insert

erythropoietin (epoa Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-rac2 guide

Plasmid

#107600

Purpose

An entry vector with U6a and U6c promoter driving rac2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

rac2 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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H2B-MiniR

Plasmid

#208328

Purpose

MiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

H2B-MiniR

Tags

MiniR

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-6

Plasmid

#172741

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-ndufs2 guide

Plasmid

#107601

Purpose

An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

ndufs2 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold-Tubulin-C-18

Plasmid

#158008

Purpose

Visualization of tubulin in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Tubulin (TUBA1B Human)

Tags

mGold

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Blasto

Plasmid

#167864

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUAST-GFP-RpL10Ab

Plasmid

#71729

Purpose

Expression of Drosophila melanogaster Rpl10Ab-GFP fusion

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Tags

EGFP

Expression

Insect

Promoter

hsp70

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
mEmerald-MyosinIIA-C-18

Plasmid

#54190

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

Insert

MyosinIIA

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pFA6a-kanMX6-P3nmt1-3HA

Plasmid

#39283

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pUb dRxCas13d + RxCas13d guide RNA

Plasmid

#176304

Purpose

Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

RxCas13d

Tags

HA tag

Expression

Insect

Mutation

R239A, H244A, R858A, H863A

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ar1

Plasmid

#225219

Purpose

Expression androgen receptor 1 in mammalian cells

Depositor

Deshou Wang

Article

Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

Insert

androgen receptor 1

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIC292

Plasmid

#51405

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Article

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska2 IMAGE 4685972 (SKA2 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pKeratin-miRFP670nano

Plasmid

#127437

Purpose

Keratin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

Keratin-miRFP670nano

Tags

miRFP670nano

Expression

Mammalian

Mutation

Keratin V282L - please see depositor comments bel…

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSunS

Plasmid

#171672

Purpose

SunS expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pWJB30

Plasmid

#126505

Purpose

Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis

Tags

8xHis

Expression

Bacterial

Promoter

PBAD30

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR H1-miR-30 L1-R5

Plasmid

#62181

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Gateway Cloning and RNAi

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

Plasmid

#121750

Purpose

pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

3x HA epitope tag + polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC72

Plasmid

#118866

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease, mGFP, cleavable…

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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