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pHAGE-BRAF-V600L

Plasmid

#116207

Purpose

Lentiviral expression of BRAF V600L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600L

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600Ifs*13

Plasmid

#116198

Purpose

Lentiviral expression of BRAF V600Ifs*13

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600Ifs*13

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.G466A

Plasmid

#81707

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway Cloning

Mutation

G466A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.G466E

Plasmid

#81715

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway Cloning

Mutation

G466E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GALNT14_p.R336Q

Plasmid

#81577

Purpose

Gateway Donor vector containing GALNT14 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GALNT14 (GALNT14 Human)

Use

Gateway Cloning

Mutation

L4V; R336Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GALNT14_p.F285L

Plasmid

#81550

Purpose

Gateway Donor vector containing GALNT14 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GALNT14 (GALNT14 Human)

Use

Gateway Cloning

Mutation

L4V; F285L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GALNT14_p.P484S

Plasmid

#81490

Purpose

Gateway Donor vector containing GALNT14 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GALNT14 (GALNT14 Human)

Use

Gateway Cloning

Mutation

L4V; P484S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GALNT14_p.A187T

Plasmid

#81475

Purpose

Gateway Donor vector containing GALNT14 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GALNT14 (GALNT14 Human)

Use

Gateway Cloning

Mutation

L4V; A187T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

luciferase

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-BRAF-V600E

Plasmid

#15269

Purpose

Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BRAF (BRAF Human)

Use

Retroviral

Tags

His and Myc

Expression

Mammalian

Mutation

Valine 600 to Glutamate (mutation has been found …

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-spikeRBD::∆ACE2 SURF

Plasmid

#206957

Purpose

Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

Depositor

Xiaokun Shu

Article

Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

Inserts

sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
ACE2 (ACE2 Human)

Tags

cSURF, mCherry, and nSURF

Expression

Mammalian

Mutation

17-615 and RBD (319-541)

Promoter

CMV

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLENTI_hACE2_HygR

Plasmid

#155296

Purpose

Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

Depositor

Raffaele De Francesco

Article

Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

Insert

hACE2 (ACE2 Human)

Use

Lentiviral

Tags

C9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-HIPK2

Plasmid

#23481

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HIPK2 (HIPK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Anti-Histone H3.3 [N158/28R]

Plasmid

#182106

Purpose

Mammalian Expression Plasmid of anti-Histone H3.3 (Rat). Derived from hybridoma N158/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Histone H3.3 (Rattus norvegicus) recombinant mouse monoclonal antibody (H3f3b Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_EML4_BRAF

Plasmid

#205812

Purpose

Express mEGFP-tagged fusion protein, EML4_BRAF from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

EML4_BRAF (EML4, BRAF Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIRREL3-Fc(DAPA)-AviTag-6xHis

Plasmid

#156896

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIRREL3 (KIRREL3 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-3'-MBD5-FLAG

Plasmid

#244261

Purpose

3' AAVLINK plasmid for MBD5 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

MBD5 (MBD5 Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLCKO-CMV-ACE2-P2A-Blasticidin

Plasmid

#237476

Purpose

Encodes full length human ACE2 protein, along with blasticidin selection marker.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Tags

P2A-Blasticidin

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Beta-2 adrenergic receptor [N430/30R]

Plasmid

#220412

Purpose

Mammalian Expression Plasmid of anti-Beta_2 adrenergic receptor (Mouse) IgG2a R-mAb. Derived from hybridoma N430/30

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Beta-2 adrenergic receptor (Mus musculus) recombinant mouse monoclonal antibody. (Adrb2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

Plasmid

#216871

Purpose

This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

Depositor

Ori Bar-Nur

Article

Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:

Insert

Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)

Use

CRISPR

Mutation

mdx mutation in mouse dystrophin

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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