We narrowed to 10,900 results for: CHL

1. pTRIP-SFFV-EGFP-NLS

   Plasmid

   #86677

   Purpose

   Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   EGFP-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24a-VHH-APEX2

   Plasmid

   #109420

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC015 - dLwCas13a-NF

   Plasmid

   #91905

   Purpose

   Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

   Expression

   Mammalian

   Mutation

   R474A and R1046A

   Promoter

   CAG

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol1-UAS:CoChR-tdTomato

   Plasmid

   #124233

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   CoChR-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24a-VHH-myc

   Plasmid

   #109422

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217515

   Purpose

   Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-CreERT2 stuffer v3

   Plasmid

   #158046

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTRIP-CMV-GFP-FLAG-cGAS

   Plasmid

   #86675

   Purpose

   Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   GFP-FLAG-cGAS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-2xflox-BRX-eGFP-NLS

   Plasmid

   #217535

   Purpose

   AAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivo

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   4X BRE reporter and miniXon casette (Smad1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   BMP response element (BRE)

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TetON-Bmpr1A-ca

    Plasmid

    #217536

    Purpose

    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    constitutively active BMP receptor 1a (BMPR1A Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet operon (TetO)

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET24a-VHH-mCherry

    Plasmid

    #109421

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p206-Switch-ON

    Plasmid

    #217885

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC034 - LwCas13a-msfGFP-2A-Blast

    Plasmid

    #91924

    Purpose

    Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol1-UAS:ChR2(H134R)-tdTomato

    Plasmid

    #124237

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    ChR2(H134R)-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO-Cre stuffer v4

    Plasmid

    #158032

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pTRIP-CMV-tagRFP-FLAG-cGAS

    Plasmid

    #86676

    Purpose

    Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    tagRFP-FLAG-cGAS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-OSBP-PH

    Plasmid

    #49571

    Purpose

    expresses the plekstrin homology domain of OSBP in mammalian cells

    Depositor

    Marci Scidmore

    Article

    Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

    Insert

    OSBP-PH (AA 91-179) (OSBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR-Cre stuffer v4

    Plasmid

    #158033

    Purpose

    Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lyn FAK biosensor

    Plasmid

    #78299

    Purpose

    FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Lyn Fak biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1 gfaABC1D-Ezr-BioID2-HA

    Plasmid

    #176744

    Purpose

    Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Ezr-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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