We narrowed to 43,183 results for: cha

1. pcDNA SF-HERC2 F1

   Plasmid

   #55784

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

2. CLC-EGFP

   Plasmid

   #193566

   Purpose

   Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   Clathrin light chain B (CLTB Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

   Plasmid

   #52494

   Purpose

   Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Science. 2014 Mar 27.

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E90R, D156N, T159C

   Promoter

   human synapsin-1 and ribosomal skip sequence

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA SF-HERC2 F3

   Plasmid

   #55786

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA SF-HERC2 F6

   Plasmid

   #55789

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

   Plasmid

   #106464

   Purpose

   Expresses Prc1 with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   PRC1
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   PRC1

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA SF-HERC2 F5

   Plasmid

   #55788

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (3550-4450aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAAV Syn ChR E90R-T159C 2A tDimer

   Plasmid

   #52495

   Purpose

   Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Science. 2014 Mar 27.

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E90R, T159C

   Promoter

   human synapsin-1 and ribosomal skip sequence

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAC8_GWs-Lox (VE5589)

   Plasmid

   #163769

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   no insert
   

   Use

   Tags

   no tag

   Expression

   Insect

   Mutation

   Promoter

   PH

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEMHE:hTRAAK(Q258K)

    Plasmid

    #133077

    Purpose

    X. laevis expression vector. It will generate the human TRAAK channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Q258K

    Promoter

    T7

    Available since

    Oct. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAC8_MF (VE5596)

    Plasmid

    #139768

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    PH or p10

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS306Sup35R1

    Plasmid

    #17715

    Depositor

    Susan Lindquist

    Article

    Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

    Insert

    pRS306Sup35R1 (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    An pRS306 based integrating plasmid designed for …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

    Plasmid

    #153446

    Purpose

    E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKP115 (pRS406-YBR139Wp-YBR139W-GFP-ADH1t)

    Plasmid

    #106465

    Purpose

    Expresses Atg42/Ybr139w with a C-terminal GFP tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t)

    Plasmid

    #106471

    Purpose

    Expresses Prc1 with a C-terminal PA tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    PRC1
    

    Use

    Tags

    PA

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    PRC1

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPicZ:mTREK-1_cryst_I110D

    Plasmid

    #209640

    Purpose

    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP

    Depositor

    Dan Minor

    Article

    Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant
    

    Use

    Tags

    SNS- 3C_cleavage_site-TAAA-GFP

    Expression

    Yeast

    Mutation

    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pILGFPEasy9R

    Plasmid

    #218291

    Purpose

    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ShadowY-CLC

    Plasmid

    #193560

    Purpose

    Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    ShadowY

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CLC-ShadowY

    Plasmid

    #193561

    Purpose

    Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    ShadowY

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTSK405

    Plasmid

    #190817

    Purpose

    For C-terminus 3xGFP tagging of a gene of interest using URA3 selection marker in S. cerevisiae

    Depositor

    Tatiana Karpova, Gunjan Mehta

    Article

    Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

    Insert

    URA3 (URA3 Budding Yeast)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge