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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2561 - 2580 of 5523 results

  4. pEvolvR-mCherry-NNG-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249079
    Purpose
    Expresses nucleus-localized NNG-Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    NNG-Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249079-plasmid-map-sequence-id-496312

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  5. pNDMS239

    Plasmid
    #248096
    Purpose
    mScarlet-I fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    mScarlet-I
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248096-plasmid-map-sequence-id-495375

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  6. pNDMS237

    Plasmid
    #248095
    Purpose
    sfYFP fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfYFP
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248095-plasmid-map-sequence-id-495374

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  7. pNDMS238

    Plasmid
    #248094
    Purpose
    sfCFP fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfCFP
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248094-plasmid-map-sequence-id-495373

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  13. L4857 dsRedExpress2 reporter, VEGFR2 NTEVp, VEGFR1 CTEVp in PiggyBac Transposon Vector

    Plasmid
    #244187
    Purpose
    PiggyBac transposon vector for expression of dsRed-Express2 synTF promoter; constitutive expression of VEGFR2 NTEVp chain, VEGFR1 CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
    Depositor
    Joshua Leonard
    Publication
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    dsRed-Express2 under synTF responsive promoter; VEGFR2 NTEVp chain with WT NTEVp; VEGFR1 CTEVp chain; mNeonGreen-P2A-PuroR
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244187-plasmid-map-sequence-id-489486

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Showing: 2561 - 2580 of 5523 results