We narrowed to 7,753 results for: 11
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Plasmid#55193PurposeA carboxyl-terminal YFP fragment was fused to Ggamma-11. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertYFP(159-238)-gamma-11 (GNG11 Aequorea victoria, Bovine)
TagsYFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationGgamma-11 was amplified by PCR, which added a Bam…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP(1-158)-gamma-11 in pcDNAI/Amp
Plasmid#55620PurposeAn amino-terminal CFP fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCFP(1-158)-gamma-11 (GNG11 Aequorea victoria, Bovine)
TagsCFP(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-11 was amplified by PCR, which added a Bam…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-11 in pcDNAI/Amp
Plasmid#55628PurposeAn N-terminal mCerulean fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-11 (GNG11 Aequorea victoria, Bovine)
TagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-11 was amplified by PCR, which added a Bam…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (1-517, WT, MG-38-11)
Plasmid#110748PurposeTransient transfection of human USP30DepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172503PurposeEncodes the DisCas7-11 protein only for bacterial recombinant protein expression for purificationDepositorInsertDisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone
Plasmid#216155PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic splice site and C-terminal FLAG tag
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDF0352 HvsCas7-11 with C-term Nes mammalian expression
Plasmid#172516PurposeEncodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cellsDepositorInsertC-term Nes HvsCas7-11 mammalian expression
ExpressionMammalianAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172505PurposeEncodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purificationDepositorInsertCjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11
Plasmid#193752Purposehuman codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGHDepositorInsertEF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide
Plasmid#186993PurposeAAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guideDepositorInsertcas7-11-s1006-gggs-d122, non-targeting crRNA guide
UseAAVMutations1006-gggs-d1221 deletionAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc
Plasmid#186987PurposeAAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guideDepositorInsertcas7-11-s1006-gggs-d122, Gluc crRNA guide
UseAAVMutations1006-gggs-d1221 deletionAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide
Plasmid#186991PurposeAAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guideDepositorInsertcas7-11-r1045-gggs-r1122, non-targeting crRNA guide
UseAAVMutationr1045-gggs-r1122 deletionAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc
Plasmid#186985PurposeAAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of Gluc crRNA guideDepositorInsertcas7-11-r1045-gggs-r1122, Gluc crRNA guide
UseAAVMutationr1045-gggs-r1122 deletionAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides
Plasmid#172506PurposeEncodes DisCas7-11 protein alone with a golden gate site for spacer cloningDepositorInsertDisCas7-11 bacterial expression vector with golden gate site for guides
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold
Plasmid#186981Purposemammalian expression of Cas7-11 crRNA guideDepositorInsertmature DR guide
ExpressionMammalianAvailable SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer
Plasmid#172501PurposeEncodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacersDepositorInsertDisCas7-11 full locus with Golden gate acceptor site for spacer
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain
Plasmid#183396PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11
UseRetroviralTagsMycPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
MoClo Plant Parts III: Transformation & Genome Engineering Kit
Plasmid Kit#1000000236PurposeCollection of MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR parts, and parts for modular geminiviral replicon assembly.DepositorApplicationCloning and Synthetic BiologyVector TypePlant ExpressionCloning TypeGolden Gate (MoClo)Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Antibody#218107-rAbPurposeAnti-V5 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to GKPIPNPLLGLDST sequence.DepositorRecommended ApplicationsWestern BlotSource SpeciesRabbitIsotypeIgGTrial SizeNot available to purchaseAvailable SinceMay 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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Yeast GoldenBraid Cloning System and Toolkit
Plasmid Kit#1000000138PurposeThis toolkit allows generation of transcriptional units using GoldenBraid for integration in the S. cerevisiae genome at loci, YPRCΔ15 and YORWΔ22, to support high and stable transgene expression.DepositorApplicationCloning and Synthetic BiologyVector TypeYeast ExpressionCloning TypeGolden Gate (GoldenBraid)Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only