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Plasmid#70660PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backboneDepositorInsertsgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2 RB1 sgRNA #2
Plasmid#202519PurposeExpressing Cas9 and sgRNA targeting human RB1 geneDepositorInsertRB1 sgRNA (RB1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18
Plasmid#164436PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHDM-SARS-CoV-2 Δ18
Plasmid#164434PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)2
Plasmid#127521PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CRISPR-V2-gYap-2
Plasmid#166482PurposeExpresses spCas9 and a gRNA targeting mouse YapDepositorInsertgRNA for mouse Yap (Yap1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CRISPRv2-sgIl1r1_#2-hygro
Plasmid#231990PurposeKnockout mouse Il1r1DepositorInsertsgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
eIF3k sgRNA-2 PX459 plasmid
Plasmid#192256Purposeexpressing sgRNA-2 targeting eIF3k locus closed to stop codenDepositorInsertsgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3f sgRNA-2 PX459 plasmid
Plasmid#192254Purposeexpressing sgRNA-2 targeting eIF3f locus closed to stop codenDepositorInsertsgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b sgRNA-2 PX459 plasmid
Plasmid#192250Purposeexpressing sgRNA-2 targeting eIF3b locus closed to stop codenDepositorInsertsgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SARS-CoV-2 S1 RBD
Plasmid#167013PurposeGateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseTagsExpressionMammalianMutationNo stop codonPromoterAvailable sinceApril 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC
Plasmid#13006DepositorInserthuman sulfatase 2 deltaCC (SULF2 Human)
UseTagsExpressionMammalianMutationchanged Cysteine 88 to AlaninePromoterAvailable sinceOct. 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - Amplicon, BCR/ABL sgRNA 2
Plasmid#70658PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a
Plasmid#213401PurposePlant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursorDepositorInsertArabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 prodomain-VC155
Plasmid#49261PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 prodomain (CASP2 Human)
UseTagsHA and split Venus CExpressionMammalianMutationdelete aa 148-435PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBIFC-flag-hCaspase 2 prodomain-VN173
Plasmid#49262PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 prodomain (CASP2 Human)
UseTagsflag and split Venus NExpressionMammalianMutationdelete aa 148-435PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 CARD-VC155
Plasmid#49259PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertCaspase-2 CARD (CASP2 Human)
UseTagsHA and split Venus CExpressionMammalianMutationdeleted aa 123-435PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-flag-hCaspase 2 CARD-VN173
Plasmid#49260PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 CARD (CASP2 Human)
UseTagsFlag and split Venus NExpressionMammalianMutationdelete aa 123-435PromoterCMVAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163 QQ4xSS #2
Plasmid#127195PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 QQ4xSS #2 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationQQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-ATG13 (2-197)
Plasmid#188075PurposeTo make ATG101/ATG13 HORMA complex for expression in mammalian cellsDepositorInsertATG13 (ATG13 Human)
UseTagsstrep-strep-Flag tagExpressionMammalianMutationATG13 (2-197)PromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only