Addgene

We narrowed to 2,447 results for: PGK

Showing: 241 - 260 of 2447 results

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pGK:HygroR-CMV:mTagBFP2-A_UTR

Plasmid

#68479

Purpose

Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mTagBFP2-A_UTR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

shRNA sensor cassette "A" added to 3&#x…

Promoter

CMV and pGK

Available since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::ZeoR-bGHpA

Plasmid

#62442

Purpose

MXS_chaining vector with PGK::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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PB-GG-OMKSL-PGK-Puro

Plasmid

#102894

Purpose

PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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PB-GG-OCT4-1-5-PGK-Puro

Plasmid

#102893

Purpose

PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OCT4-gRNAs-1-5-PGK-puro (POU5F1 )

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Flag-MFF-WT

Plasmid

#74379

Purpose

Lentiviral expression vector containing flag tagged WT MFF isoform 5

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pENTR-L1-PGK-TKBsd-L2

Plasmid

#141009

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

TKBsd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEN-X-hPGK-PuroR-Z

Plasmid

#218904

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-PuroR

Use

Tags

Expression

Mammalian

Mutation

246T>A and 398T>C

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(PGK)

Plasmid

#172328

Purpose

LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Mutation

Promoter

PGK

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-hKLF4

Plasmid

#60435

Purpose

Over-expression of human KLF4

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

KLF4 (KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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PGK1 gRNA (BRDN0001144730)

Plasmid

#77980

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PGK1 gRNA (BRDN0001487049)

Plasmid

#77981

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-PGK-Chst3

Plasmid

#193024

Purpose

AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter

Depositor

Bart Nieuwenhuis

Article

Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.103

Insert

Chst3 (Chst3 Mouse)

Use

AAV

Tags

3xFLAG and myc-tag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::tTA2-bGHpA

Plasmid

#62448

Purpose

MXS_chaining vector with PGK::tTA2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSin wPGK-Cre

Plasmid

#101242

Purpose

low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Cre recombinase

Use

Lentiviral

Tags

Expression

Mutation

see attached file

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-pGK:GFP

Plasmid

#68486

Purpose

Retrovirus expressing GFP driven by pGK promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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PGK1 gRNA (BRDN0001146969)

Plasmid

#77982

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v6 (-cyt)

Plasmid

#137817

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6 (-cyt) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region, R296K (please see dep…

Promoter

PGK

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha variant 2, G318A

Plasmid

#188365

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE:shRNA A-pGK:rtTA-Cre

Plasmid

#68471

Purpose

Inducible lentivirus expressing an arbitrary shRNA and Cre

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

GFP-shRNA A-miR30
rtTA-2A-Cre

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

pGK and pTRE

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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