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pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L1-#1

Plasmid

#171503

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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E42_control_NGFR

Plasmid

#189800

Purpose

Retroviral delivery of control guide RNA

Depositor

Ellen Rothenberg

Article

Hosokawa et al Nat Immunol. 2018 Dec;19(12):1427-1440.

Insert

Luciferase gRNA

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DRB

Plasmid

#164992

Purpose

Expression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DRB1*01:02:01

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TYMS

Plasmid

#183326

Purpose

All-in-One CRISPRko system with a guide RNA that targets TYMS gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TYMS

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_KDR

Plasmid

#183305

Purpose

All-in-One CRISPRko system with a guide RNA that targets KDR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

KDR

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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1046E=dgRNAs[traB,bTub]

Plasmid

#149425

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

U6.3-gRNAs[TraB, bTub]

Use

CRISPR

Expression

Insect

Promoter

U6.3

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAftsI

Plasmid

#149649

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAftsI

Plasmid

#149650

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAftsI

Plasmid

#149563

Purpose

ftsI gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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sg1

Plasmid

#113966

Purpose

Single short guide RNA targeting GTATAGCATACATTATACG

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-CMV-Luc

Plasmid

#133367

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PIR702W-Luc

Plasmid

#133362

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IRFP-702

Tags

HA-tagged IRFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-Luc

Plasmid

#133360

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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gh144

Plasmid

#106803

Purpose

expression of gRNA targeting LARGE

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

LARGE

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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TRIN-shMYB.2652

Plasmid

#105575

Purpose

Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB shRNA #2652

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone1

Plasmid

#162125

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-shWT1_B

Plasmid

#217637

Purpose

Dox inducible shRNA targeting WT-1

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shWT1_B (WT1 Human)

Use

RNAi

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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