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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,492 results for: LEA

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Showing: 2581 - 2600 of 12492 results

  1. GFP-BLM DM

    Plasmid
    #80071
    Purpose
    Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations
    Depositor
    Nathan Ellis
    Publication
    Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BLM (BLM Human)
    Tags
    GFP-BLM
    Expression
    Mammalian
    Mutation
    K317R (AAA>AGA) and K331R (AAA>AGA)
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80071-plasmid-map-sequence-id-150335

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  3. pcDNA3-AktAR

    Plasmid
    #61624
    Purpose
    FRET-based biosensor for monitoring Akt activity
    Depositor
    Jin Zhang
    Publication
    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AktAR (AKT1 Human, Synthetic, Budding Yeast)
    Tags
    Cerulean and cpVenus[E172]
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61624-plasmid-map-sequence-id-100425

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  4. pMM288

    Plasmid
    #46036
    Depositor
    Raymond Monnat
    Publication
    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WRN E84A (WRN Human)
    Use
    Tre, bidirectional promoter
    Tags
    5x myc
    Expression
    Mammalian
    Mutation
    E84A exonuclease-dead mutant; S2G introduced duri…
    Promoter
    CMV2
    Available since
    May 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    46036-plasmid-map-sequence-id-64898

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  6. pcDNA3-Akt-STOPS-NLS

    Plasmid
    #175007
    Purpose
    Nuclear-targeted Akt-STOPS.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Akt-STOPS-NLS
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    175007-plasmid-map-sequence-id-344969

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  9. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92114-plasmid-map-sequence-id-197615

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  11. SpCas9-HF1

    Plasmid
    #138556
    Purpose
    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138556-plasmid-map-sequence-id-274763

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  20. pMM289

    Plasmid
    #46037
    Depositor
    Raymond Monnat
    Publication
    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WRN E84A/K577M (WRN Human)
    Use
    Tre, bidirectional promoter
    Tags
    5x myc
    Expression
    Mammalian
    Mutation
    E84A/K577M exonuclease and helicase-dead mutant; …
    Promoter
    CMV2
    Available since
    May 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    46037-plasmid-map-sequence-id-64899

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Showing: 2581 - 2600 of 12492 results