We narrowed to 3,926 results for: SPL

1. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

   Plasmid

   #207634

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5HT2C-RNA1-FLAG

   Plasmid

   #79678

   Purpose

   serotonin receptor 2C RNA isoform 1 with C-terminal flag tag

   Depositor

   Stefan Stamm

   Article

   Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

   Insert

   5HT2C-RNA1 (HTR2C Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

   Plasmid

   #172984

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE-UBC-hCD24-T2A-GFP

   Plasmid

   #205456

   Purpose

   lentiviral plasmid for expression of human CD24

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD24 (CD24 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-IFT43

   Plasmid

   #218735

   Purpose

   Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

   Insert

   IFT43 (IFT43 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcS-E626-C1C2

   Plasmid

   #200162

   Purpose

   This plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

   Depositor

   Masako Harada

   Article

   Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

   Inserts

   E626
   C1C2 domain of lactadherin
   

   Use

   Tags

   HA and HIS

   Expression

   Mammalian

   Mutation

   sequence adopted from doi: 10.1371/journal.pone.0…

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_nV5-AMOT (p130)

   Plasmid

   #172997

   Purpose

   constitutive expression of V5-tagged AMOT (p130)

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOT (p130) (AMOT Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_nV5-AMOTL1

   Plasmid

   #172998

   Purpose

   constitutive expression of V5-tagged AMOTL1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOTL1 (AMOTL1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-neo-TET

   Plasmid

   #51286

   Purpose

   expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   U6 (RNU6-1 Human)
   

   Use

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Mutation

   Promoter

   U6 upstream enhancer sequence

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRE-UBC-hCD46-T2A-GFP

    Plasmid

    #205457

    Purpose

    lentiviral plasmid for expression of human CD46

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46 (CD46 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-shSNAI1

    Plasmid

    #115467

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shSNAI1 (SNAI1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRE-UBC-Qa1 SCT-T2A-GFP

    Plasmid

    #205453

    Purpose

    lentiviral plasmid for expression of mouse Qa1 SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    Qa1 SCT (H2-T23 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

    Plasmid

    #216763

    Purpose

    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-PQBP1

    Plasmid

    #25804

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Apr 21. ():.

    Insert

    PQBP1 (PQBP1 Human)
    

    Use

    Tags

    6xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pEGFP_sfCherry2(11)_Clathrin light chain

    Plasmid

    #83032

    Purpose

    Expresses sfCherry2(11) tagged clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_Clathrin light chain (CLTA Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P-1-SF2

    Plasmid

    #99020

    Purpose

    Bacterial expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-RNF138-WT

    Plasmid

    #78920

    Purpose

    Mammalian expression of RNF138 with an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

18. CaMKIV-dCT

    Plasmid

    #126422

    Purpose

    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMCB320-sgNC.SPA

    Plasmid

    #169834

    Purpose

    Expresses a negative control sgRNA

    Depositor

    Laura Attardi

    Article

    Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

    Insert

    safe harbor sgRNA
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA flag-Msi1

    Plasmid

    #184029

    Purpose

    Msi1 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Msi1 (Msi1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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