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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE-neo largeTgenomic

Plasmid

#10891

Depositor

William Hahn

Insert

large T and small T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Article

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pmVenus(L68V)-mTurquoise2

Plasmid

#60493

Purpose

Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

SYFP2-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA wt

Plasmid

#19298

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pEFmEGFP-mDia2

Plasmid

#25407

Depositor

Arthur Alberts

Article

Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

Insert

mDia2 (Diaph3 Mouse)

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Encodes mDia2

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-RhoA

Plasmid

#23224

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoA (RHOA Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad2

Plasmid

#14042

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA cyclin D1 HA

Plasmid

#11181

Depositor

Bruce Zetter

Article

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-1 myc

Plasmid

#11374

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-1 (Irs1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-N1

Plasmid

#79988

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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PPRE-pNL1.3

Plasmid

#84394

Purpose

Secreted Luciferase reporter for PPARgamma activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Tags

secNluc

Expression

Mammalian

Promoter

PPRE

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pX330 Pten

Plasmid

#59909

Purpose

pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Article

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting Pten (Pten Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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H2B-Venus

Plasmid

#20971

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Tags

Venus

Expression

Mammalian

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-RBBP4

Plasmid

#20715

Depositor

Yue Xiong

Article

He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

Insert

retinoblastoma binding protein 4 (RBBP4 Human)

Tags

myc3

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC 75L

Plasmid

#19305

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Tags

GST and HA

Expression

Mammalian

Mutation

S75L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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hTLR2 flag

Plasmid

#13082

Depositor

Ruslan Medzhitov

Insert

TLR2 (TLR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-16 have been replaced with mammalia…

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5 (w71-3)

Plasmid

#17425

Purpose

Gateway entry vector for N-term V5 tag, frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe HcRed

Plasmid

#10677

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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