We narrowed to 32,159 results for: uros

1. pLKO-puro-tetON-shOGDH 2956

   Plasmid

   #110943

   Purpose

   Tet-inducible shRNA targeting OGDH

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shOGDH (OGDH Human)
   

   Use

   Lentiviral and RNAi

   Promoter

   H1/TO

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-hGFAP-NEUROD1-T2A-mCherry

   Plasmid

   #178582

   Purpose

   Expression of NEUROD1 and mCherry under the human GFAP promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   NEUROD1 (NEUROD1 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-HA

   Plasmid

   #162109

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to StrepTagII-ProtC-HA
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-HA

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-puro GSK3 beta (S9A)

   Plasmid

   #14128

   Depositor

   Brendan Manning

   Article

   Zhang et al Mol Cell. 2006 Oct 20. 24(2):185-97.

   Insert

   GSK3 beta (S9A) (GSK3B Human)
   

   Use

   Retroviral

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   S9A. Mutant version of GSK3 that lacks the inhibi…

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pdCas9-DNMT3A-PuroR (ANV)_v2

   Plasmid

   #74408

   Purpose

   Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   CBh

   Available Since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1272 pBabe puroL Myr HA Akt3

   Plasmid

   #9019

   Depositor

   William Sellers

   Insert

   Akt3 (AKT3 Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

7. XLone-Puro SPI1 P2A mCherry

   Plasmid

   #140029

   Purpose

   Tunable and temporal expression control of SPI1 and mCherry

   Depositor

   Xiaoping Bao

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-puro-Venus-FLAG-CD59

   Plasmid

   #50377

   Purpose

   Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cells

   Depositor

   Reika Watanabe

   Article

   Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

   Insert

   Venus
   

   Expression

   Mammalian

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMXs-puro GFP-p62 L343A

   Plasmid

   #38279

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Sequestosome-1 (Sqstm1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed L343A

   Available Since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-SlugCBE-NNG-P2A-puro

    Plasmid

    #214355

    Purpose

    Expresses SlugCBE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCBE-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5 HA3-eDHFR-T2A-puro

    Plasmid

    #86395

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A puro
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A puro

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid

    #71829

    Purpose

    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEpicVector3mC_Puro-T2A-mCherry-ProtC-HA-FLAG

    Plasmid

    #162115

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to ProtC-HA-FLAG
    

    Use

    Lentiviral

    Tags

    ProtC-HA-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-FLAG

    Plasmid

    #162086

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-HA-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-HA-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-AU1

    Plasmid

    #162093

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-HA-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-HA-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-AU1

    Plasmid

    #162096

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to ProtC-HA-AU1
    

    Use

    Lentiviral

    Tags

    ProtC-HA-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-HA

    Plasmid

    #162083

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-ProtC-HA
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-HA

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-IRES-Puro PFKM

    Plasmid

    #201638

    Purpose

    Over-expression of untagged human PFKM

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PFKM (PFKM Human)
    

    Use

    Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-EF1a-GFP-miRE_shRen-Puro

    Plasmid

    #135683

    Purpose

    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLG1-puro Non-targeting sgRNA 3

    Plasmid

    #109003

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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