We narrowed to 23,581 results for: CHI

1. pUCmini-iCAP-AAV.MaCPNS2

   Plasmid

   #185137

   Purpose

   non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

   Insert

   Synthetic construct isolate AAV-MaCPNS2 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-IDO1-p2a-eGFP

   Plasmid

   #187026

   Purpose

   Expresses IDO1 and GFP (seperately) in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

   Insert

   Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
   

   Use

   Tags

   P2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

   Plasmid

   #216164

   Purpose

   Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   Gaussia princeps luciferase (with double mutation to improve stability)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSIIpuro-AREG-ScNeo

   Plasmid

   #209897

   Purpose

   To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   AREG-ScNeo (AREG Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mScarlet

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSIIpuro-EPGN-ScNeo

   Plasmid

   #209899

   Purpose

   To monitor the status of Epigen, the plasmid encodes a recombinant Epigen fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   EPGN-ScNeo (EPGN Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mScarlet

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSIIpuro-EGF-ScNeo

   Plasmid

   #209893

   Purpose

   To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   EGF-ScNeo (EGF Human)
   

   Use

   Lentiviral

   Tags

   GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSIIpuro-HBEGF-ScNeo

   Plasmid

   #209894

   Purpose

   To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   HBEGF-ScNeo (HBEGF Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mScarlet

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSIIpuro-EREG-ScNeo

   Plasmid

   #209896

   Purpose

   To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   EREG-ScNeo (EREG Human)
   

   Use

   Lentiviral

   Tags

   GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHLS-EF1a-FRB-SpCas9-A

   Plasmid

   #138477

   Purpose

   Expresses FRB fused SpCas9 for NanoMEDIC packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   SpCas9, human codon optimized
   

   Use

   CRISPR

   Tags

   3x HA tag and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TDP-REGv1(mCherry-FLAG) in pTwist-CMV

    Plasmid

    #216165

    Purpose

    Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mCherry with C-terminal FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TDP-REGv2 Cryptic PE-Max

    Plasmid

    #216163

    Purpose

    Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    PEMax for prime editing with internal cryptic exon
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-BACE1-mBFP

    Plasmid

    #196697

    Purpose

    Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Beta-secretase 1 (BACE1 Human)
    

    Use

    Tags

    HA and mBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pelB-MBP-BB0238-ss

    Plasmid

    #137046

    Purpose

    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature BB0238: lacks the BB0238 signal peptide

    Promoter

    T7lac

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTR-UF11

    Plasmid

    #157970

    Purpose

    AAV vector plasmid containing the Chimeric CMV/Chicken Beta Actin promoter driving 'humanized' GFP

    Depositor

    Sergei Zolotukhin

    Article

    Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10

    Has Service

    AAV.A01.T, AAV.A02.T, AAV.A03.T, AAV.A04.T, AAV.A05.T, AAV.A06.T, AAV.A07.T, AAV.A08.T, AAV.A09.T, AAV.A10.T, AAV.A11.T, and AAV.A12.T

    Insert

    'humanized' e.g. codon optimized GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    chimeric CMV/Chicken Beta actin (CBA)

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXS-IRES-Blast mAtg7

    Plasmid

    #173170

    Purpose

    Retroviral vector to express sgRNA resistant mouse Atg7

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pelB-MBP-P66-ss

    Plasmid

    #137045

    Purpose

    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66949.1 (p66 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature P66: lacks the P66 signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRetroX-TRE3G-PLOD2_PGK-GpNLuc

    Plasmid

    #136457

    Purpose

    Expresses Tet/Dox-inducible human PLOD2 in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

    Insert

    TRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pelB-MBP-P13-ss

    Plasmid

    #137065

    Purpose

    E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature P13: lacks the P13 signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCIneoLuc-TEAD4

    Plasmid

    #128335

    Purpose

    Vector for LUMIER assay

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Insert

    TEAD4 (TEAD4 Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W

    Plasmid

    #208548

    Purpose

    Lentiviral vector expressing gRNA targeting human GATA3

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GATA3(13) (GATA3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge