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pJAM1.16_PB-Cx43_S368A-PGK-Blast

Plasmid

#205561

Purpose

Express human Cx43 with S368A mutation

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Cx43

Use

Piggybac

Expression

Mammalian

Mutation

S368A

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-NINJ1

Plasmid

#201179

Purpose

Expression of NINJ1 gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Use

Retroviral

Tags

Flag

Promoter

MESV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2pA2-drl:APEX2-mCherry

Plasmid

#188945

Purpose

Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

Depositor

Owen Tamplin

Article

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA

Use

Tol2 destination vector (attr4-r3)

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_SIN3A_PAH

Plasmid

#178486

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SIN3A_PAH (SIN3A Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II-EGFP

Plasmid

#120316

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-NpHR3.3-EYFP (AAV8)

Viral Prep

#137151-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-nEF-NpHR3.3-EYFP (#137151). In addition to the viral particles, you will also receive purified pAAV-nEF-NpHR3.3-EYFP plasmid DNA. nEF-driven expression of NpHR3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Promoter

nEF

Tags

EYFP

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only
FLAG-FBXL17

Plasmid

#236188

Purpose

Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FLAG-FBXL17 (FBXL17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Rtn2b-GFP

Plasmid

#186599

Purpose

Encodes human full-length Reticulon 2 isoform B fused to GFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-mCherry

Plasmid

#186600

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHRi_1G4 hTCRα

Plasmid

#187603

Purpose

Lentiviral expression of hTCR alpha (1G4) for low expression

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

1G4 hTCR alpha (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mHSP

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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tagSrc(WT)-mCer3 S208F/V224L

Plasmid

#179138

Purpose

mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA

Tags

mCerulean3 (S208F/V224L)

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PUM1_PUM_pGEX

Plasmid

#135129

Purpose

Recombinant expression of PUM1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PUM1 (PUM1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)

Plasmid

#136543

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Tags

ANXA2 (23-339) and His-tag

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670

Plasmid

#82621

Purpose

Expresses NLS-tdiRFP670 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670

Use

Piggybac vector

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI036

Plasmid

#105350

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

LexA operator & 35S min promoter

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorA(sp)

Plasmid

#111253

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptide

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorA signal peptide

Expression

Bacterial

Promoter

Pc

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET30a-SRAP-delCys2

Plasmid

#84568

Purpose

Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Srap1 (Hmces Mouse)

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

deletion of Cys2

Promoter

T7

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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tdEos-Vimentin-7

Plasmid

#57688

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Vimentin (VIM Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-Zyxin-6

Plasmid

#57441

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Zyxin (ZYX Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PX544 SpCas9(del1098-1368)

Plasmid

#58889

Purpose

Truncated form of SpCas9 without C' domain

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 1098-1368

Available Since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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