We narrowed to 15,851 results for: ef1
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Plasmid#216480Purposelentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excisionDepositorInsertsUseCre/Lox and LentiviralMutationS66A, S89A, S117A, S311APromoterEF1aL and UBCAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCDH-EF1-DIO-Nluc-copGFP-IRES-Puro
Plasmid#192875PurposeCre-dependent dual reporterDepositorInsertNanoluc-P2A-copGFP
UseLentiviralAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
Plasmid#176630PurposeIntegrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell linesDepositorInsertEF1a-TET3G-P2A-ERT2-Gal4-BGHpA
UseSynthetic BiologyAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH
Plasmid#74289PurposepAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent mannerDepositorInsertH2B-GFP and oG (optimized Glycoprotein)
UseAAVExpressionMammalianMutationchimeric glycoproteinPromoterEf1aAvailable SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP
Plasmid#35509PurposeAAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.DepositorHas ServiceAAV5 and AAV9InserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123T and T159CPromoterEf1aAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP
Plasmid#118594PurposeParental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertsgRNA/Puro-T2A-BFP
UseLentiviralExpressionMammalianPromoterEF1AlphaAvailable SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]
Plasmid#227245PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyExpressionMammalianPromoterEF1α and pTRE3GAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP 2.0
Plasmid#126081PurposevCre-Dependent ChR2(H134R)-eYFP with the vLox sites in the reverse orientationDepositorInserthChR2(H134R)-eYFP
UseAAVTagseYFPMutationH134RPromoterEf1aAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo
Plasmid#120555PurposeExpress Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cellsDepositorInsertCas9, NES, ERT2 and GCN4
UseLentiviralExpressionMammalianAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP
Plasmid#170327PurposeDouble-floxed inverse Orientation HA and IRES-YFP tagged RPL22DepositorAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA
Plasmid#47457PurposeLITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.DepositorInsertNLS(alpha-imp)-CRY2PHR-NLS-VP64
Tags2A_GFPExpressionMammalianPromoterEF1-alphaAvailable SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB
Plasmid#169921PurposeDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.DepositorInsertIRES-Puro-pEF1a-EGFP
UseCRISPRPromoterEF1aAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL
Plasmid#217676PurposeAAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.DepositorInsertGtACR2-P2A-Voltron2ST
UseAAVTagsSoma targeting sequence (Kv2.1) on Voltron2 gene …ExpressionMammalianPromoterEF‐1αAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR
Plasmid#24584DepositorInsertpADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…Available SinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73032PurposeExpress fluorescent and luminescence dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseExpressionMammalianPromoterTruncated EF1Available SinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)
Plasmid#73476PurposeEncodes the G protein to enable transsynaptic RAV infectionDepositorInsertH2B-GFP-P2A-N2c(G)
UseAAVExpressionMammalianAvailable SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1
Plasmid#153062PurposeLentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)DepositorInsertTruncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
UseLentiviralExpressionMammalianMutationMutagenesis to insert a stop codon at aa 298 in B…PromoterEF1αAvailable SinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry
Plasmid#83491Purposecre-dependent DN-AMPK under EF1a promoterDepositorInsertDN-AMPK-2A-mCherry
UseAAV and Cre/LoxExpressionMammalianMutationK45RAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE
Plasmid#130997PurposeDouble floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoterDepositorInsertChRmine
UseAAVTagsEYFP-Kv2.1PromoterEf1aAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTY-EF1a-mKdm2b (del CxxC domain)-Flag
Plasmid#61740PurposeExpress CxxC domain deleted mouse histone demethylase Kdm2bDepositorAvailable SinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only