We narrowed to 7,296 results for: ef1a

1. pMIA3-Keppel

   Plasmid

   #214446

   Purpose

   Expresses eSpCas9 and gRNA targeting Keppel-19 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-pCaMKII-tTAc

   Plasmid

   #172125

   Purpose

   Expression of InteinC-tTAC in CaMKII positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   pCaMKII, tTAc
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

   Plasmid

   #180587

   Purpose

   FdCd

   Depositor

   Won Do Heo

   Article

   Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_831

   Plasmid

   #216100

   Purpose

   CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMIA3-Olônne

   Plasmid

   #214447

   Purpose

   Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSH231-EFS-Cas9-BlastR

   Plasmid

   #115146

   Purpose

   Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette

   Depositor

   Michael Phelps, Raymond Monnat

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   Cas9-T2A-BlastR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMV_AA088

   Plasmid

   #216104

   Purpose

   Knockout, EF1a-driven Cas9 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

   Plasmid

   #197893

   Purpose

   Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   EF1a

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

   Plasmid

   #178575

   Purpose

   Lentiviral vector for GFAP::Opto-vTrap

   Depositor

   Won Do Heo

   Article

   Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

   Insert

   CRY2PHR-P2A-CIBN-mCherry-VAMP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMV_AA090

    Plasmid

    #216099

    Purpose

    Knockout, EF1a-driven Cas12a (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-SAM-hygro

    Plasmid

    #138955

    Purpose

    Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

    Depositor

    Nadya Dimitrova

    Article

    Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 AND EF1a

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV V5-LAP2beta

    Plasmid

    #175112

    Purpose

    Lentiviral expression of V5-tagged mouse Tmpo

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmpo (Tmpo Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

    Plasmid

    #201560

    Purpose

    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR and EGFP
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pIND(SP1)

    Available since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWPXL-Hes1

    Plasmid

    #36983

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Hes1 (Hes1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. AA065

    Plasmid

    #215965

    Purpose

    Fragmid fragment: (Pol 2 promoter) human EF1a promoter

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    EF1a_v1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pONSY-CoNMM:mCherry

    Plasmid

    #111878

    Purpose

    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSH231-EF1-BLST-Cas9-VPR

    Plasmid

    #115147

    Purpose

    Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

    Depositor

    Michael Phelps, Raymond Monnat

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    BlastR-P2A-Cas9-VPR
    

    Use

    CRISPR

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1α-M2rtTA-T2A-Puro

    Plasmid

    #169175

    Purpose

    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

    Depositor

    Igor Slukvin

    Article

    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

    Insert

    M2rtTA-T2A-Puro
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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