We narrowed to 12,861 results for: sequence

1. pT5T_hp53_ST_CR2

   Plasmid

   #34923

   Depositor

   Thanos Halazonetis

   Article

   Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

   Insert

   p53 DNA-binding (Res 94-358, deletion 293-321) (TP53 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

   Promoter

   T7

   Available since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. FCER1a-bio-His

   Plasmid

   #51878

   Purpose

   Expresses full-length High affinity immunoglobulin epsilon receptor subunit alpha ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   FCER1a (FCER1A Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. ss-ED-mCherry-GPI

   Plasmid

   #163680

   Purpose

   Exprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   The luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences (ST6GAL1 Human)
   

   Use

   Tags

   GPI anchor, mCherry, and signal sequences

   Expression

   Mammalian

   Mutation

   This chimera contains the ecto-domain of ST. Its …

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-ecDHFR-N23PP/L28F/G51PEKN

   Plasmid

   #118585

   Purpose

   Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23, L28 mutated to F28, G51 mutat…

   Promoter

   T7

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRTTO-NHASt-codoptUL50

   Plasmid

   #135101

   Purpose

   Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL50 codon optimized
   

   Use

   Tags

   HA-Strep-Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE_puro_ZFP64_N_3XFLAG

   Plasmid

   #118693

   Purpose

   Express 3xflag tagged ZFP64 for flag ChIP experiments

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Insert

   ZFP64 (ZFP64 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiV_Neo_WT_ZFP64

   Plasmid

   #118661

   Purpose

   Express human wild type ZFP64 gene

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Insert

   ZFP64 (ZFP64 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPICZ-pre-Ost1-alphaf(I)-Btl2

   Plasmid

   #117667

   Purpose

   Study secretion efficiency of Btl2

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-Ost1-alphaf(I)-Btl2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the lipase Btl2 together wit…

   Promoter

   pAOX1

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. RBM6_RRM_pGEX

   Plasmid

   #135157

   Purpose

   Recombinant expression of RBM6 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBM6 (RBM6 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GGH-bio-His

    Plasmid

    #52066

    Purpose

    Expresses full-length Gamma-glutamyl hydrolase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    GGH (GGH Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.3 HA-TSPAN12^11-LEL

    Plasmid

    #115785

    Purpose

    Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    large extracellular loop of TSPAN12 replaced with…

    Promoter

    CMV

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. L309-myc-seipin-ΔCT

    Plasmid

    #64062

    Purpose

    myc-tagged murine seipin with deletion of cytosolic C-terminus); lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

    Insert

    bscl2 (Bscl2 Mouse)
    

    Use

    Lentiviral

    Tags

    myc epitope

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal Asp338–Ser443

    Promoter

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

13. IGFALS-bio-His

    Plasmid

    #52180

    Purpose

    Expresses full-length Insulin-like growth factor-binding protein complex ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    IGFALS (IGFALS Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5FRTTO-NHASt-codoptUL23

    Plasmid

    #135104

    Purpose

    Codon-optimized HSV-1 UL23 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL23 codon optimized
    

    Use

    Tags

    HA-Strep-Strep (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5FRTTO-codoptUL23-CStHA

    Plasmid

    #135099

    Purpose

    Codon-optimized HSV-1 UL23 with C-terminal StrepHA tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL23 codon optimized
    

    Use

    Tags

    Strep-Strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LMAN2-bio-His

    Plasmid

    #51923

    Purpose

    Expresses full-length Vesicular integral-membrane protein VIP36 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LMAN2 (LMAN2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CD59-bio-His

    Plasmid

    #53154

    Purpose

    Expresses full-length CD59 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD59 (CD59 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BOLL_RRM_pGEX

    Plasmid

    #135130

    Purpose

    Recombinant expression of BOLL with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    BOLL (BOLL Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PPBP-bio-His

    Plasmid

    #52074

    Purpose

    Expresses full-length Platelet basic protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PPBP (PPBP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1a_KLF12_P2A_Hygro_Barcode

    Plasmid

    #120498

    Purpose

    Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF12 (KLF12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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