We narrowed to 2,618 results for: ubiquitin

1. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211843

   Purpose

   Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

   Plasmid

   #211839

   Purpose

   Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Plant

   Mutation

   R181A R185A

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211841

   Purpose

   Plant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA and mCherry

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSC6

   Plasmid

   #104780

   Purpose

   pSC218UG-At Ubiquitin10:Gateway expression cassette binary vector

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC5

   Plasmid

   #104779

   Purpose

   pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUB-Cas9

   Plasmid

   #86556

   Purpose

   For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

   Depositor

   Andreas Weber

   Article

   Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Cas9 is codon optimized for C. reinhardtii

   Promoter

   UBIQUITIN10

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPAtUbq10 (GB0223)

   Plasmid

   #68174

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq10 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTAtUbq3 (GB0219)

   Plasmid

   #68192

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq3 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA066

   Plasmid

   #215966

   Purpose

   Fragmid fragment: (Pol 2 promoter) human UbiquitinC promoter

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   UbC_v1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211844

    Purpose

    Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVUHshp53-tTR-KRAB

    Plasmid

    #11654

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. ppBAalbNixE1E2 PUb-GFP

    Plasmid

    #173667

    Purpose

    transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

    Inserts

    Nix masculinisation gene
    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Nix isoforms with only exons 1, 2 separated by in…

    Promoter

    Aedes aegypti Polyubiquitin and cloned Nix promot…

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. OA-986F (AAEL003877-dCas9-VPR)

    Plasmid

    #183993

    Purpose

    Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    AAEL003877-dCAS9-VPR
    

    Use

    CRISPR

    Tags

    dCas9-VPR

    Expression

    Insect

    Mutation

    Promoter

    AAEL003887

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUb-tdTomato-LP-Neo-LP

    Plasmid

    #231509

    Purpose

    Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system

    Depositor

    Yeonchul Hong

    Insert

    tdTomato fluorescent protein
    

    Use

    Unspecified; Acanthamoeba castellanii neff strain

    Tags

    Expression

    Mutation

    Promoter

    Acanthamoeba polyubiquitin

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVUTHshGATA1-tTR-KRAB

    Plasmid

    #11650

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUTKan-3xHA-GFP-TOM5

    Plasmid

    #130674

    Purpose

    Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

    Depositor

    Andreas Weber

    Article

    Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

    Insert

    Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
    

    Use

    Tags

    3xHA and sGFP

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin10

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX3

    Plasmid

    #183904

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB PUb-integrase

    Plasmid

    #183966

    Purpose

    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    PhiC31 integrase
    

    Use

    Piggybac insect transgenesis destination vector

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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