We narrowed to 30,498 results for: Ide

1. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEF1-alpha-V

   Plasmid

   #27290

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin alpha V (ITGAV Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Isoform 2

   Promoter

   Available since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEvolvR-enCas9-PolI3M-TBD

   Plasmid

   #113077

   Purpose

   Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI3M-TBD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p8092 LentiCRISPR v2 sgNT-1

   Plasmid

   #163315

   Purpose

   Expresses Cas9 and a non-targeting control guide RNA

   Depositor

   Elizabeth White

   Article

   Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

   Insert

   sgNT-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #50946

   Purpose

   An empty gRNA expression vector

   Depositor

   Kosuke Yusa

   Article

   Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LTR5HS S. pyogenes scaffold CARGO array

   Plasmid

   #191316

   Purpose

   Contains an array of guide RNAs targeting LTR5Hs elements

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. nontarget S. pyogenes scaffold CARGO array

   Plasmid

   #191319

   Purpose

   Contains an array of nontargeting guide RNAs

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NLS-SpCas9mSA-His

   Plasmid

   #182951

   Purpose

   Express SpCas9mSA in bacteria

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   NLS-SpCas9mSA-His
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

    Plasmid

    #74317

    Purpose

    A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish

    Depositor

    Martin Meyer, Stephen Smith

    Article

    Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

    Inserts

    synaptophysin
    DsRedExpress
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    5UAS

    Available since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-mSA*

    Plasmid

    #182952

    Purpose

    Express SpCas9-mSA*

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    SpCas9-mSA*
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUbC-nls-ha-stdMCP-stdGFP

    Plasmid

    #98916

    Purpose

    synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

    Depositor

    Robert Singer

    Article

    Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

    Insert

    stdMCP-stdGFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 560HTR

    Plasmid

    #24444

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

14. Human PNP his-tagged

    Plasmid

    #64076

    Purpose

    Expression of human Purine Nucleoside Phosphorylase

    Depositor

    Vern Schramm

    Article

    Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pET28a-Ec.coaBC (pCLK1210)

    Plasmid

    #50448

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

    Insert

    PPCS PPCDC
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Flag TSC2

    Plasmid

    #14129

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEBTet_NADP-Snifit-cyto

    Plasmid

    #113917

    Purpose

    Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TetO2

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET14-GFP30_Encap

    Plasmid

    #86406

    Purpose

    Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 30aa CLP tag
    Encapsulin
    

    Use

    Tags

    6x Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET14-GFP15_Encap

    Plasmid

    #86407

    Purpose

    Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 15aa CLP tag
    Encapsulin
    

    Use

    Tags

    6x Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBTet_NADP-Snifit-mito

    Plasmid

    #113919

    Purpose

    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TetO2

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge