We narrowed to 10,582 results for: plasmids 101

1. pAAV-Ef1a-Coff/Fon-oScarlet

   Plasmid

   #137138

   Purpose

   Intersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-oScarlet
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E95D

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-Coff/Fon-sRGECO

   Plasmid

   #137128

   Purpose

   Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-sRGECO
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E217D

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

   Plasmid

   #137165

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   3x-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-Con/Foff 2.0-BFP

   Plasmid

   #137130

   Purpose

   Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

   Plasmid

   #127867

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse, Human)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   constructed by blunt end fusion of the 5’ 51 nucl…

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

   Plasmid

   #174134

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   Only contains the sequences coding for amino acid…

   Promoter

   hSyn

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

   Plasmid

   #197892

   Purpose

   Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   hM4D/2A/GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   EF1a

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIS1 ATP2B1

   Plasmid

   #60788

   Purpose

   Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and wild-type miR-155 sites

   Depositor

   David Bartel

   Article

   Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

   Insert

   ATP2B1 3'UTR and wild-type miR-155 binding site (ATP2B1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   thymidine kinase (HSV-TK promoter)

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. R619-M68-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP

   Plasmid

   #166019

   Purpose

   mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S )
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIS1 LMBRD2

    Plasmid

    #60800

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains LMBRD2 3' UTR and wild-type miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    LMBRD2 3'UTR and wild-type miR-155 binding site (LMBRD2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. human ETV6 gRNA-4

    Plasmid

    #133404

    Purpose

    human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon.

    Depositor

    Linzhao Cheng

    Article

    Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

    Insert

    ETV6 (ETV6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOGS539_GLuc

    Plasmid

    #226718

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Gaussia Luciferase
    

    Use

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

    Plasmid

    #215026

    Purpose

    Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    4X E-box mutations within Coordinator motifs

    Promoter

    SV40

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

    Plasmid

    #174133

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Only contains the sequences coding for amino acid…

    Promoter

    hSyn

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

    Plasmid

    #174135

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Only contains the sequences coding for amino acid…

    Promoter

    hSyn

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

    Plasmid

    #166020

    Purpose

    mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S )
    

    Use

    Tags

    3C-His8-SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFnCpf1GG

    Plasmid

    #131010

    Purpose

    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of FnCas12a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pK.myc-MYPT1

    Plasmid

    #24101

    Depositor

    Masumi Eto

    Article

    Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

    Insert

    Myosin-targeting subunit-1 (PPP1R12A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S50G, P415Q

    Promoter

    Available Since

    March 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. PLKO.1-G3BP1-CDS

    Plasmid

    #136039

    Purpose

    G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCerulean-Keratin-17

    Plasmid

    #55379

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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