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peCALWY-NB

Plasmid

#22239

Depositor

Maarten Merkx

Publication

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY non-binding variant

Expression

Mammalian

Mutation

C284S, C287S, C413S and C416S mutations linker c…

Available since

Jan. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pCBFD1/HBFD1Bad

Plasmid

#53364

Purpose

Will convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd1

Expression

Bacterial

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Publication

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Available since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-GfaABC1D-GRAB_Ado1.0

Plasmid

#153288

Purpose

Express the adenosine sensor GRAB_Ado1.0 in astrocytes

Depositor

Yulong Li

Publication

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

GfaABC1D

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

Plasmid

#101299

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Publication

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-698 and 698-906 of alpha-C…

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX317-SNAI1

Plasmid

#115446

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

SNAI1 (SNAI1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pCDB180

Plasmid

#80677

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

Depositor

David Baker

Publication

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Type

Empty backbone

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-ARF

Plasmid

#19929

Depositor

Yue Xiong

Publication

Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

Insert

cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)

Expression

Bacterial

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1

Plasmid

#10880

Depositor

Patrick Aebischer

Publication

Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

Insert

H1-shSOD1 (SOD1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pBAD-mhYFP

Plasmid

#186532

Purpose

Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-S147P/L195M/V206K

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-EPHB6

Plasmid

#23931

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHB6 (EPHB6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
PB_SBE4:mScarlet-I-NLS

Plasmid

#154961

Purpose

encodes transcriptional reporter of SMAD2/3

Depositor

Ewa Bielczyk-Maczynska, Joshua Knowles

Publication

Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

Insert

mScarlet

Use

Piggybac

Tags

NLS-PEST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-CALM1

Plasmid

#23768

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CALM1 (CALM1 Human)

Use

Gateway Cloning

Mutation

M74T V144E

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pEX-CMV-SP-STIM2(15-746)

Plasmid

#18868

Depositor

Tobias Meyer

Publication

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

STIM2 (STIM2 Human)

Expression

Mammalian

Mutation

Signal peptide(SP) 1-10 from STIM1 and residues 1…

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Publication

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Publication

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mEos4a-C1

Plasmid

#54810

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Publication

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4a

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBCN22-R4R3

Plasmid

#26302

Depositor

Ben Lehner

Publication

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Type

Empty backbone

Expression

Worm

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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TU#710

Plasmid

#15278

Depositor

Martin Chalfie

Publication

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzgfp

Expression

Worm

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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