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pcDNA3-T11HA-hMOV10-CD

Plasmid

#178908

Purpose

expression of catalytic dead hMOV10 K530A

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

cDNA Catalytic dead hMOV10 (MOV10 Human)

Tags

T11 beta strand of GFP-HA

Expression

Mammalian

Mutation

K530A catalytic dead AAG>GCA

Promoter

CMV protmoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Soat2_Luc

Plasmid

#177623

Purpose

Cloned human Soat2 promoter containing exon1 and 2 coupled to luciferase

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

human Soat2

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVuncs-mTagBFP2-HA

Plasmid

#178869

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVuncs-mTagBFP-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVuncs(NEYLQFL)-mTagBFP2-HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate8

Plasmid

#165005

Purpose

Expression of candidate TCR #8 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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VTEHDTLLY_epitope

Plasmid

#165017

Purpose

Expression of CMV UL44 (245-253) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (245-253)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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NTTVTFLPTPSLILQ_epitope

Plasmid

#165020

Purpose

Expression of CMV UL44 (37-51) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (37-51)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

Plasmid

#162811

Purpose

Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_(C1A)-Intein_DD

Use

Piggybac

Expression

Mammalian

Mutation

Intein(Cys1,Ala)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D977A

Plasmid

#129674

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

D977A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ290-E848A

Plasmid

#129675

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Tags

E848A; AacCas12b is rice codon optimized

Expression

Plant

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_HsPDZ1b

Plasmid

#131216

Purpose

Expresses HsPDZ1b (Homo sapiens) folllwed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

HsPDZ1b

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_HsPDZ1b_X

Plasmid

#131214

Purpose

Expresses HsPDZ1b (Homo sapiens) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

HsPDZ1b

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_AtCIB

Plasmid

#131194

Purpose

Expresses AtCIB (Arabidopsis thaliana) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtCIB

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_AtPIF6

Plasmid

#131192

Purpose

Expresses AtPIF6 (Arabidopsis thaliana) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtPIF6

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_AtCIB_X

Plasmid

#131181

Purpose

Expresses AtCIB (Arabidopsis thaliana) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtCIB

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS

Plasmid

#126203

Purpose

Mammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTR

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIC3Nme

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p1149-pCSDest-AcrIIA4-mCherry-miR122BS

Plasmid

#126205

Purpose

Mammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTR

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIA4

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAL829

Plasmid

#119697

Purpose

precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C

Tags

none

Expression

Bacterial

Mutation

mutations Y283D, G289C and V347C

Promoter

T5

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCK039

Plasmid

#105425

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

dTALE (binding to Bs3 promotor (Capsicum annuum) as transactivator)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK038

Plasmid

#105426

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

dTALE (binding to Bs3 promotor (Capsicum annuum) as transactivator)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG822

Plasmid

#105430

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

YFP fused to SV40 Nuclear Localisation Signal

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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