We narrowed to 12,675 results for: BASE;

1. pU6-GG-gRNA

   Plasmid

   #226860

   Purpose

   Human gRNA expression vector backbone with BsmBI Golden Gate site

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-BCR-ABL1-p190-IRES-EGFP

   Plasmid

   #219964

   Purpose

   Express human BCR-ABL1-P190 in mammalian cells

   Depositor

   Mohamed Fareh

   Insert

   BCR-ABL1-P190 (ABL1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p667-UBC-CDK12-V5_IDG-K

   Plasmid

   #135245

   Purpose

   Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK12-V5 (CDK12 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETZZtevPP7CP(C68A,C73A)

   Plasmid

   #27548

   Depositor

   Kathleen Collins

   Article

   Hogg et al RNA. 2007 Jun . 13(6):868-80.

   Insert

   Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
   

   Use

   Tags

   TEV protease cleavage site, six histidine tag, an…

   Expression

   Bacterial

   Mutation

   changed two cysteine to alanine (C68A,C73A) to in…

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24b-SUMO-BG2.4

   Plasmid

   #199438

   Purpose

   expresses BAmGreen2.4 in bacterial cells with a SUMO tag

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen2.4
   

   Use

   Tags

   his tag and sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVT36b

   Plasmid

   #184437

   Purpose

   CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

   Depositor

   Huimin Zhao

   Article

   Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pESC-ADE (V5 and HA)

   Plasmid

   #222489

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and ADE2 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p663-UBC-V5-GSK3B_IDG-K

   Plasmid

   #158166

   Purpose

   Gateway destination clone of V5-GSK3B (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-GSK3B (GSK3B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMU-ACTLr

   Plasmid

   #61193

   Purpose

   Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   LifeAct-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHSV-TK

    Plasmid

    #165801

    Purpose

    Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    HSV-TK
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

    Plasmid

    #134857

    Purpose

    Expresses EGFP-tagged synaptic vesicle marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 3C
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSALECT-EcoBam

    Plasmid

    #59705

    Purpose

    Folding reporter with ampicillin resistance as a readout

    Depositor

    Christopher Anderson

    Article

    Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD441_WT_hi

    Plasmid

    #128857

    Purpose

    High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CellTagB

    Plasmid

    #124592

    Purpose

    Expressed barcode for sample multiplexing in single-cell analysis

    Depositor

    Samantha Morris

    Article

    Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

    Insert

    CellTagB
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FlagMBNL3

    Plasmid

    #96901

    Purpose

    expression of Flag-tagged human MBNL3

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    human MBNL3 cDNA (MBNL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYZ1389

    Plasmid

    #215348

    Purpose

    Nla CRISPRi Delivery Vector

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    Nla CRISPRi Genes (Cas5, Cas8c, Cas7)
    

    Use

    CRISPR; Nla i delivery vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEVA13-GG

    Plasmid

    #208002

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    14G

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLTR-RVG

    Plasmid

    #17577

    Depositor

    Jakob Reiser

    Article

    Mochizuki et al J Virol. 1998 Nov . 72(11):8873-83.

    Insert

    rabies virus glycoprotein (SAD B19 strain)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-13Myc-TRP1

    Plasmid

    #41601

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    13Myc and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEX128·gRNA

    Plasmid

    #187600

    Purpose

    Template for amplification of gRNA with Cas6 recognition site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    gRNA template
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    no promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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