We narrowed to 9,603 results for: FIE

1. pLXSN-H-Ras_N17

   Plasmid

   #39537

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS N17 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N17

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

2. ETV1_pet28a

   Plasmid

   #131644

   Purpose

   Bacterial expression of ETS gene ETV1

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETV1 (ETV1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TIMP3-bio-His

   Plasmid

   #53418

   Purpose

   Expresses full-length Metalloproteinase inhibitor 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TIMP3 (TIMP3 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9-CAT

   Plasmid

   #80323

   Purpose

   Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Inserts

   Cas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mutation

   Promoter

   TgTUB1

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. RHBDD1 pEGFP-N2 (1078)

   Plasmid

   #62028

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   RHBDD1 (RHBDD1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. ETS2_pet28a

   Plasmid

   #131664

   Purpose

   Bacterial expression of ETS gene ETS2

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETS2 (ETS2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lenti-SAM-hygro

   Plasmid

   #138955

   Purpose

   Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

   Depositor

   Nadya Dimitrova

   Article

   Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 AND EF1a

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest-636

   Plasmid

   #159574

   Purpose

   Gateway Destination vector for protein expression in baculovirus

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Type

   Empty backbone

   Use

   Tags

   His6-MBP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWzyE-distrepII

   Plasmid

   #196667

   Purpose

   Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   TEV-cleavable di-strep tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

    Plasmid

    #129532

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIA4
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEasyG1_hph

    Plasmid

    #184912

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMP71-mIL15-IL12rb1-sushi-il12rb2

    Plasmid

    #174606

    Purpose

    Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MMP11-bio-His

    Plasmid

    #53408

    Purpose

    Expresses full-length Stromelysin-3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    MMP11 (MMP11 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

    Plasmid

    #202617

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    FRT-JEDI-1P-Kv
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe_puro_DEST_Flag_SKIL

    Plasmid

    #45265

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SNON (SKIL Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

18. EGFP-uTEV1-MAVS

    Plasmid

    #170998

    Purpose

    HiLITR protease with MAVS c-terminal tmd targeting information (Mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-hCMV1-hNANOG-IV

    Plasmid

    #60432

    Purpose

    tet-inducible human NANOG

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    NANOG (NANOG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SERPINA-bio-His

    Plasmid

    #52182

    Purpose

    Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINA (SERPINA1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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