We narrowed to 7,730 results for: pac

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pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L

Plasmid

#146864

Purpose

Insect Expression of DmPABPC1_del364-550-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del364-550-siRNAres (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and five silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_1-1199-del862-1115-dsRNAres_L

Plasmid

#146820

Purpose

Insect Expression of DmGW182_1-1199-del862-1115-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182_1-1199-del862-1115-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z

Plasmid

#148160

Purpose

Insect Expression of Dm4E-T_1-511-LLFWKtoAAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)

Expression

Insect

Mutation

alias: 4E-T; one non silent mutation L127S, annot…

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_AC

Plasmid

#148466

Purpose

Insect Expression of DmNanos_116-163-E151AF152AN1555A

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-E151AF152AN1555A (nos Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2-del393-429-W556E-dsRNAres_U

Plasmid

#147698

Purpose

Insect Expression of DmNot2-del393-429-W556E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del393-429-W556E-dsRNAres (Rga Fly)

Expression

Insect

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-510-Y10AL15A-V5His6_D

Plasmid

#146152

Purpose

Insect Expression of Dm4E-T_1-510-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510-Y10AL15A (4E-T Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

Plasmid

#214138

Purpose

Insect cell expression of human 20S CP alpha-subunits

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA1 (PSMA1 Human)
PSMA2 (PSMA2 Human)
PSMA3 (PSMA3 Human)
PSMA4 (PSMA4 Human)
PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)

Plasmid

#206434

Purpose

Vasa 463-661 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Vasa 463-661 (vas Fly)

Tags

EGFP

Expression

Insect

Mutation

F504E

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)

Plasmid

#206461

Purpose

Oskar 139-240 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-240 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

Plasmid

#52889

Purpose

"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

Plasmid

#101218

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N692A/M694A/Q695A/H698A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N692A, M694A, Q695A and H698A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

Plasmid

#176589

Purpose

Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associate IscB (Chesapeake Bay) locus

Expression

Bacterial

Mutation

Truncated CRISPR array to two direct repeats, rep…

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

Plasmid

#216385

Purpose

Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Norrin (NDP Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C and Rho1D4

Expression

Insect

Mutation

C93A/C95A/C131A

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Plasmid

#165493

Purpose

Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Use

AAV

Tags

darkVenus

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX

Plasmid

#202718

Purpose

Mammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Pink Flamindo
paGFP-bPAC-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Plasmid

#165492

Purpose

Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Use

AAV

Tags

darkVenus

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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VN550 pAC-cI_E34P-Asf1A_Term_f1-pOL2-62_L-eGFP-KanR

Plasmid

#235102

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

Asf1A (ASF1A Human)

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 388-606 (OSK domain) (HK75)

Plasmid

#206463

Purpose

Oskar 388-606 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 388-606 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-EGFP-Aubergine 4R-K (R11K/R13K/R15K/R17K) (HK121)

Plasmid

#206433

Purpose

Aubergine expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Aubergine (aub Fly)

Tags

EGFP

Expression

Insect

Mutation

R11K/R13K/R15K/R17K

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Plasmid

#219653

Purpose

General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus

Depositor

Thomas Oertner

Insert

2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Use

AAV

Tags

2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)

Expression

Mammalian

Promoter

nEf Addgene # 149296

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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