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Lentihair s1GFP

Plasmid

#8451

Depositor

Bob Weinberg

Publication

Stewart et al RNA 2003 Apr;9(4):493-501.

Insert

siRNA against GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pOSCAR

Plasmid

#29639

Depositor

David_L Andrews, Scott Gold

Publication

Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

Type

Empty backbone

Available since

Sept. 19, 2011

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Publication

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

Plasmid

#106204

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S + mRuby3 fusion

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DY2230AA

Plasmid

#64878

Purpose

lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

Depositor

Richard Wood

Publication

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

D2330A,Y2331A, in the DNA polymerase domain (POL)…

Promoter

EF1alpha

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSMALB-ATF4.12

Plasmid

#155033

Purpose

Fluorescent reporter for ATF4 translation, Negative Control

Depositor

John Dick, Peter van Galen

Publication

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 start codon mutated

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-DAP5-3XFLAG

Plasmid

#127331

Purpose

Express 3xFLAG tagged DAP5 protein from AUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DM

Plasmid

#64880

Purpose

lentiviral expression of human POLQ double mutant (in POL and HDL domains)

Depositor

Richard Wood

Publication

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K121M and D2330A,Y2331A

Promoter

EF1alpha

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Publication

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
pET26-MBP-GLIC

Plasmid

#20887

Depositor

Raimund Dutzler

Publication

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421

Tags

His, MBP, and pelB

Expression

Bacterial

Available since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-CASK

Plasmid

#23470

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CASK (CASK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rtTA

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUPRT::DHFR-D

Plasmid

#58528

Purpose

replace TgUPRT with DHFR by homologous recombination

Depositor

David Sibley

Publication

Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

Insert

DHFR cassette

Use

Gateway Cloning

Mutation

pyrimethamine resistant

Promoter

DHFR

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAM1573

Plasmid

#40239

Depositor

Susan Golden

Publication

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

Cm Resistance, neutral site II integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

Plasmid

#159128

Purpose

expresses KEAP1 in mammalian cells

Depositor

Michele Pagano

Publication

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KEAP1 (KEAP1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.14

Plasmid

#155034

Purpose

Fluorescent reporter for ATF4 translation, Positive Control

Depositor

John Dick, Peter van Galen

Publication

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 and uORF2 start codons mutated

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Publication

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-MAGI1

Plasmid

#23523

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAGI1 (MAGI1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pLPC TRF2 deltaB deltaM

Plasmid

#18008

Depositor

Titia de Lange

Publication

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pLL39

Plasmid

#15458

Depositor

Chia Lee

Publication

Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

Insert

Use

S. aureus-e. coli shuttle vector

Available since

Oct. 9, 2007

Availability

Academic Institutions and Nonprofits only

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