We narrowed to 51,260 results for: des.1

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pHR- flag-Lag16-NCAM-1

Plasmid

#205199

Purpose

This vector encodes of the synCAM (NCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM NCAM 1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag17-iCAM-1

Plasmid

#205204

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG17

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag17 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag6-iCAM-1

Plasmid

#205210

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG6

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag6 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag18-iCAM-1

Plasmid

#205207

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG18

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag18 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag42-iCAM-1

Plasmid

#205205

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG42

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag42 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag29-iCAM-1

Plasmid

#205208

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG29

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag29 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag43-iCAM-1

Plasmid

#205206

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG43

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag43 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6-AMPK alpha1 (1-312)

Plasmid

#162131

Purpose

Expression vector for constitutively active AMPK alpha 1

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

Insert

AMPK alpha1 (1-312) (PRKAA1 Rat)

Use

Lentiviral

Tags

V5

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-99a-WT

Plasmid

#215880

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-99a-WT (MIR99A Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-CBP(1-287)

Plasmid

#182839

Purpose

CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

CBP1-287

Expression

Bacterial

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-CenpB 1-166

Plasmid

#108474

Purpose

expression of vsv-CenpB 1-166

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB 1-166 AA (CENPB Human)

Tags

VSV

Expression

Mammalian

Mutation

encodes only the DNA-binding domain of CENP-B

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-455-WT

Plasmid

#215881

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Amber-free HIV-1 deltaRT

Plasmid

#213007

Purpose

An replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.

Depositor

Maolin Lu

Article

Ao et al Cell Chem Biol. 2024 Jan 8:S2451-9456(23

Inserts

HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
Env BG505

Use

Unspecified

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-LBR(1-245)-GFP

Plasmid

#222425

Purpose

To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(1-245aa) (LBR Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Truncation (Includes aa 1-245)

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-mTagBFP2-rMPC1 shRNA

Plasmid

#229016

Purpose

Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

MPC1 (Mpc1 Rat)

Use

Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

U6

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Galntl5 variant #1-3xHA

Plasmid

#240645

Purpose

The nucleotides coding mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.

Depositor

Taichi Noda

Article

Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:

Insert

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_WT&Mut-SM

Plasmid

#215916

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

three tandem repeats of seed-matched (SM) sequence of siBRAF_WT&Mut (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_WT&Mut-CM

Plasmid

#215920

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_WT&Mut (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-53BP1(1-1710) 3LA

Plasmid

#185698

Purpose

Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.

Depositor

Daniel Durocher

Article

Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.

Insert

53BP1(1-1710) 3LA (TP53BP1 Human)

Use

Retroviral

Tags

Flag tag, HA tag, and tetracysteine tag

Expression

Mammalian

Mutation

L175A, L517A, and L693A

Promoter

SV40

Available Since

July 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMX-53BP1(1-1710) 3LA7A

Plasmid

#185707

Purpose

Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.

Depositor

Daniel Durocher

Article

Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.

Insert

53BP1(1-1710) 3LA7A (TP53BP1 Human)

Use

Retroviral

Tags

Flag tag, HA tag, and tetracysteine tag

Expression

Mammalian

Mutation

T302A, S452A, S523A, S543A, S625A, S784A, and S89…

Promoter

SV40

Available Since

July 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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