We narrowed to 77,003 results for: rest

1. Tom20-mCherry-GAI(1-92)

   Plasmid

   #37316

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Tags

   Tom20 and mCherry

   Expression

   Mammalian

   Mutation

   contains aa 1-92

   Promoter

   CMV

   Available Since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pGLucIPZ

   Plasmid

   #228703

   Purpose

   Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

   Depositor

   Thorsten Stiewe

   Article

   Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

   Inserts

   Gaussia luciferase
   non-silencing shRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 2, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. EC71022

   Plasmid

   #159608

   Purpose

   Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   loxP-mCherry-HDEL-t35S-loxP
   

   Use

   Cre/Lox and Synthetic Biology

   Mutation

   All BsaI, Esp3I and BPiI restriction sites were r…

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDragon-empty

   Plasmid

   #155018

   Purpose

   Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

   Depositor

   Alberto Rosello-Diez

   Article

   Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

   Type

   Empty backbone

   Use

   Mouse Targeting

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

   Plasmid

   #51473

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

   Depositor

   Tobias Rose, Mark Huebener, Tobias Bonhoeffer

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Insert

   mRuby2-P2A-GCaMP6m
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xFLAG-dCas9/pMSCVpuro

   Plasmid

   #134983

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. C1-MPAct(delta1-6)-mRuby3

   Plasmid

   #155223

   Purpose

   F-actin bidning mutant of MPAct

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
   

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Deletion of first 6 AA from F-tractin (9-14 from …

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. A3Bi-Cas9n-UGI

   Plasmid

   #119135

   Purpose

   Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3B L1 intron-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   L1 intron insertion

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. C1-FT-mCitrine-EAAARx8-CaaX

   Plasmid

   #155224

   Purpose

   MPAct with increased search radius

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
   

   Tags

   mCitrine

   Expression

   Mammalian

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUPERIORpuro-shGAS5

    Plasmid

    #46370

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

    Insert

    GAS5 (GAS5 Human)
    

    Use

    RNAi

    Promoter

    H1

    Available Since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCRISPReporter

    Plasmid

    #65007

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    none

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX_970_puro_DEST_V5_TLOC1 (399aa)

    Plasmid

    #45288

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pAPcrRNA_Lmo

    Plasmid

    #126501

    Purpose

    L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCH6 - pBait-ChiX

    Plasmid

    #174662

    Purpose

    arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

    Depositor

    Katherine Berry

    Article

    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

    Insert

    ChiX
    

    Tags

    1xMS2 hairpin

    Expression

    Bacterial

    Promoter

    pBad

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. psiCHECK2-miR-34 MT

    Plasmid

    #78259

    Purpose

    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    mutated miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Expression

    Mammalian

    Mutation

    nucleotides 2-5 and 10-11 changed from CCGT to GG…

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lyn-CFP-GAI(1-92)

    Plasmid

    #37311

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pFBD-Akt1(WT)

    Plasmid

    #86561

    Purpose

    Expresses human full-length Akt1(WT)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIND-CLucZ

    Plasmid

    #53224

    Purpose

    Inducible expression of Cypridina luciferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Cypridina luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 26, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pZ005 (AacC2c1 human codon optimized protein)

    Plasmid

    #70171

    Purpose

    Expresses AacC2c1 human codon optimized protein

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    AacC2c1 protein
    

    Tags

    NLS

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFKBPmCherry-Akt2(K14A)

    Plasmid

    #86597

    Purpose

    Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    FKBB-mCherry-Akt2(K14A) (AKT2 )
    

    Expression

    Mammalian

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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