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pBJ035_MTB_gtACR2_KGC_eGFP_ER2

Plasmid

#209278

Purpose

Ubiquitously expresses gtACR2 in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

gtACR2_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ037_MTB_FR-GECO1c

Plasmid

#209279

Purpose

Ubiquitously expresses FR-GECO1c in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

FR-GECO1c

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF- RPA4 (MSW#716)

Plasmid

#208077

Purpose

Expression of human Replication Protein A 4 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4 (RPA4 Human)

Expression

Bacterial

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pJV0016

Plasmid

#205133

Purpose

Expression of 2xPX-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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UBE2G2 shRNA

Plasmid

#201813

Purpose

siRNA

Depositor

Allan Weissman

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

Insert

UBE2G2 (UBE2G2 Human)

Use

RNAi

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a no GFP)

Plasmid

#172339

Purpose

mnucTomato

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a)TetR

Plasmid

#172352

Purpose

Tet Repressor reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

TetR
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pL8N_RB610-HBB_X1

Plasmid

#191521

Purpose

Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAC, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE RB610-HBB X1

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pB7_9-ND51_v8_X1

Plasmid

#191511

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE 9-ND51 X1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pT7PI-Sce ESARC D326A

Plasmid

#195609

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

T3593C, A3601C, T3602G

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCZGY2748

Plasmid

#193331

Purpose

Site specific CRISPR/Cas9 editing of C. elegans Chr I

Depositor

Yishi Jin

Article

Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

Insert

sgRNA for ttTi4348

Tags

None

Expression

Worm

Promoter

U6

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pL6_3xG34-DCAF8L2_X1

Plasmid

#191518

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG34-DCAF8L2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pL5N_LT32-HBB_X1

Plasmid

#191520

Purpose

Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAN, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE LT32-HBB X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pL7_3xG35-EMILIN2_X1

Plasmid

#191517

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG35-EMILIN2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pB6_R13-ND51_v8_X1

Plasmid

#191512

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE R13-ND51 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-420

Plasmid

#192993

Purpose

Plasmid that encodes for Hydra Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE32

Plasmid

#90374

Purpose

SP1 - constitutive enhancer gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

SP1

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE11

Plasmid

#90345

Purpose

SRF - Serum response factor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

SRF

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB280

Plasmid

#185073

Purpose

Larger fusion of Nma111 nuclear localization signal to GFP

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111 aa 1-83

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB282

Plasmid

#185074

Purpose

Smaller fusion of Nma111 nuclear localization signal to GFP

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111 aa 1-35

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB465

Plasmid

#185098

Purpose

NMA111-GFP with NLS1 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS1-GFP

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB466

Plasmid

#185099

Purpose

NMA111-GFP with NLS1 and NLS2 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS1DNLS2-GFP

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB467

Plasmid

#185100

Purpose

NMA111-GFP with NLS2 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS2-GFP

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22U6CatalE4PCh(#767)

Plasmid

#184078

Purpose

cyclofen-inducible Catalase activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Insert

CatalDeltaC-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes) + in vitro tra…

Mutation

CatalDeltaC: deleted 516-527; ERT2: G400V, M543A,…

Promoter

Zf-Ubi+SP6

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT-AIDm-EGFP

Plasmid

#182044

Purpose

in vitro transcription of AIDm-EGFP

Depositor

Janice Evans

Article

Camlin et al Biol Reprod. 2019 Oct 25;101(4):704-718.

Insert

AIDm-EGFP

Use

In vitro transcription

Tags

EGFP

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-GFP-harbour-seal-Caspase-1/4b

Plasmid

#183368

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4b

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Cdc42

Plasmid

#180582

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH2157

Plasmid

#179204

Purpose

Pacr-2::Ca -insensitive D3cpv unc-54 3' UTR C.elegans neural expression of Pacr-2 calcium D3cpv

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

D3cpv

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-miRFP670-Zdk1-MCS

Plasmid

#178873

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of miRFP670-Zdk1

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

miRFP670-Zdk1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS181

Plasmid

#177637

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

HaloTag

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS212

Plasmid

#177639

Purpose

In vitro transcription of Patiria miniata Lamp1

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Lamp1

Use

In vitro transcription

Tags

mCherry

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS217

Plasmid

#177640

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS221

Plasmid

#177643

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K57A, K58A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS222

Plasmid

#177644

Purpose

In vitro transcription of Patiria miniata Lamp1

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Lamp1

Use

In vitro transcription

Tags

3xGFP

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS218

Plasmid

#177641

Purpose

In vitro transcription of Patiria miniata Rab7

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Rab7

Use

In vitro transcription

Tags

mCherry

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS202

Plasmid

#177638

Purpose

In vitro transcription of Patiria miniata Frizzled 1

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Frizzled 1

Use

In vitro transcription

Tags

GFP

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-AcrIIC3

Plasmid

#174036

Purpose

In vitro transcription of anti-CRISPR AcrIIC3 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC3

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-AcrIIC1

Plasmid

#174035

Purpose

In vitro transcription of anti-CRISPR AcrIIC1 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC1

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5441 - 5480 of 26655 results

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