Addgene

We narrowed to 9,727 results for: Ada;

Showing: 2721 - 2740 of 9727 results

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pPB-cT3G-cERP2-DMRT1

Plasmid

#222571

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMRT1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMRT1 (DMRT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DMRTC2

Plasmid

#222548

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMRTC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMRTC2 (DMRTC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MEIOSIN

Plasmid

#222551

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MEIOSIN

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MEIOSIN (LOC388553 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DPPA3

Plasmid

#222578

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DPPA3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DPPA3 (DPPA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-CTCFL

Plasmid

#222546

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of CTCFL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

CTCFL (CTCFL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-3xFLAG-SMAD1-active

Plasmid

#222579

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD1 (SMAD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

S487E, S489E

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-3xFLAG-SMAD9-active

Plasmid

#222580

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD9

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD9 (SMAD9 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

S489E, S491E

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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T. aq sigmaA

Plasmid

#215350

Purpose

Express T. aq sigmaA transcription initiation factor in E. coli cells

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

rpoD

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA TR KO 2

Plasmid

#207610

Purpose

sgRNA 2 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

TCAGGCCGCAGGAAGAGGAA

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA TR KO 1

Plasmid

#207609

Purpose

sgRNA 1 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

ACCCTAACTGAGAAGGGCGT

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCIwt-PRM-Wasabi

Plasmid

#195553

Purpose

Always-on expression of mWasabi reporter with transcription activation by wildtype CI

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
CIwt

Use

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB1-PGK-EGFP

Plasmid

#204018

Purpose

Homologous donor template for NFKB1 knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB1-PGK-tdTomato

Plasmid

#204019

Purpose

Homologous donor template for NFKB1 knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB2-PGK-EGFP

Plasmid

#204020

Purpose

Homologous donor template for NFKB2 knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB2-PGK-tdTomato

Plasmid

#204021

Purpose

Homologous donor template for NFKB2 knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2-HR1bR181A R185A-GFP-L (B3-B4)

Plasmid

#211857

Purpose

Mutant E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Tags

GFP

Expression

Mutation

R181A R185A

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)

Plasmid

#211863

Purpose

Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA

Expression

Mutation

C492A

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5k-AcrAB-AsLOV2*(543)

Plasmid

#210863

Purpose

IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Inserts

acrA
acrB

Use

Synthetic Biology

Tags

AsLOV2*(543)

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHFdist_rm

Plasmid

#162318

Purpose

P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

IHF distal site is removed

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-sTagRFP-Zeo

Plasmid

#207734

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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