We narrowed to 6,278 results for: Cre expression vector

1. TFORF1100

   Plasmid

   #143924

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GTF2IRD1 (GTF2IRD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRL-VinculinVenus-S1033A

   Plasmid

   #211896

   Purpose

   Vinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinVenus-S1033A (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   VenusA206K

   Expression

   Mutation

   mutated vinculin serine 1033 to alanine (S1033A),…

   Promoter

   CMV

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-VinculinVenus-S1033D

   Plasmid

   #211897

   Purpose

   Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinVenus-S1033D (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   VenusA206K

   Expression

   Mutation

   mutated vinculin serine 1033 to aspartic acid (S1…

   Promoter

   CMV

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINDUCER20_mStrawberry_Atg4BC74A

   Plasmid

   #161735

   Purpose

   Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   220-222 TGC (Cys) is changed to GCA (Ala)

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPC-dnMAML1-mVenus

   Plasmid

   #171023

   Purpose

   Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

   Depositor

   Masashi Narita

   Article

   Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

   Insert

   MAML1 Dominant Negative (MAML1 Human)
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   amino acids 12-74 only, hence conferring dominant…

   Promoter

   CMV

   Available since

   April 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLIK_Hyg_mTurquoise2_Atg4BC74A

   Plasmid

   #161733

   Purpose

   Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   220-222 TGC (Cys) is changed to GCA (Ala)

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

   Plasmid

   #169189

   Purpose

   Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

   Insert

   3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   3xFlag-His6

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

   Plasmid

   #169185

   Purpose

   Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   His6-3xFlag (nsp12 C-terminus)

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

   Plasmid

   #169180

   Purpose

   Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp7-His6-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   His6 (internal tag linking nsp7 and nsp8): nsp7-H…

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid

    #169183

    Purpose

    Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag (nsp12 C-terminus) and His6 (as internal t…

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid

    #169181

    Purpose

    Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    No purification tag and nsp7-nsp8 fusion protein …

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169162

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    6His-3xFlag

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

    Plasmid

    #169163

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPTK007-3a-αMFΔ

    Plasmid

    #84966

    Purpose

    Type 3a, α-mating factor pre-sequence shortened - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    α-mating factor, pre-sequence shortened secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPTK008-3a-αMFΔ no Kex

    Plasmid

    #84967

    Purpose

    Type 3a, α-mating factor no Kex recognition site - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    α-mating factor no Kex recognition site secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MKC106_HBB(HBA1reg-HBA1-IRES-tEPOR)

    Plasmid

    #232411

    Purpose

    AAV production plasmid for HBA+tEPOR vector from Figs. 4-6 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA+tEPOR cassette.

    Depositor

    Kyle Cromer

    Article

    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

    Insert

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MKC62_HBB(HBA1reg-HBA1-2A-YFP)

    Plasmid

    #232408

    Purpose

    AAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.

    Depositor

    Kyle Cromer

    Article

    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

    Insert

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-EmGFP-LATS2/1 KD

    Plasmid

    #52085

    Purpose

    Lentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporter

    Depositor

    Yutaka Hata

    Article

    Yang et al Mol Cell Biol. 2014 Feb 18.

    Insert

    LATS2 and LATS1 miRNAs (LATS1, LATS2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ATG-1929

    Plasmid

    #108712

    Purpose

    CBR2opt in pF4Ag expression vector with T7 and CMV promoter

    Depositor

    Keith Wood

    Article

    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

    Insert

    CBR2opt
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promega used directed evolution to create CBR (Cl…

    Promoter

    T7, CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2TAL3-RR

    Plasmid

    #37276

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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