We narrowed to 16,180 results for: Uty

1. pET28-MRPP1

   Plasmid

   #67862

   Purpose

   bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

   Depositor

   Walter Rossmanith

   Article

   Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

   Insert

   MRPP1 (TRMT10C Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   mitoch. form (contains aa 40-403)

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. rat Kv3.1-HA

   Plasmid

   #113055

   Purpose

   Expresses rat Kv3.1 in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

   Insert

   rat Kv3.1 (Kcnc1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SG3ΔENV CA Q63A,Q67A

   Plasmid

   #145784

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA Q63A,Q67A).

   Depositor

   Wesley Sundquist

   Article

   Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

   Insert

   Capsid
   

   Use

   Lentiviral and Retroviral

   Expression

   Mammalian

   Mutation

   Changed Capsid residues Glutamines 63 and 67 to a…

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGI3EM09

   Plasmid

   #135482

   Purpose

   Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   SpunHsp70pr hph-GFP ADH1t
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Promoter

   SpunHsp70pr

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Myo 1d

   Plasmid

   #134906

   Purpose

   Expression of rat Myo 1d (Myr 4) in mammalian cells.

   Depositor

   Martin Bähler

   Article

   Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

   Insert

   Myo 1d (rat) with 3' UTR (Myo1d Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mOtop1_pGEMHE

   Plasmid

   #114674

   Purpose

   This construct was used to express mouse Otop1 in Xenopus laevis oocytes

   Depositor

   Emily Liman

   Article

   Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

   Insert

   Otopetrin 1 (Otop1 Mouse)
   

   Use

   For generating the mrna, which can highly express…

   Mutation

   SNP (rs49325632): Glycine 396 to Alanine

   Promoter

   T7

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUb dRxCas13d + RxCas13d guide RNA

   Plasmid

   #176304

   Purpose

   Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   RxCas13d
   

   Tags

   HA tag

   Expression

   Insect

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   Ubi-p63e

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-Arp11 (1-417)

   Plasmid

   #51398

   Purpose

   expression of full-length mouse Arp11 in mammalian cells

   Depositor

   Trina Schroer

   Article

   Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

   Insert

   Arp11 (Actr10 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 5, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pET-28a-His6-SUMO-VAMP3

   Plasmid

   #135552

   Purpose

   Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP3
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-FLJ90652

    Plasmid

    #15359

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    FLJ90652 (INO80E Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAd-Sox2

    Plasmid

    #19767

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Science. 2008 Nov 7;322(5903):945-9. doi

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pMP71-tCD19-LLO-SIINFEKL

    Plasmid

    #174598

    Purpose

    expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    membrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEM09

    Plasmid

    #135479

    Purpose

    Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr
    

    Tags

    GFP and hph

    Expression

    Bacterial and Yeast

    Promoter

    Spun Hsp70pr

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T1-Claspin-FL-wt

    Plasmid

    #31621

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Tags

    Flag, GST, and His

    Expression

    Bacterial

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pMM9-MAPKK2-WT

    Plasmid

    #40805

    Depositor

    Natalie Ahn

    Article

    Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    MSV-LTR

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pCDH-EF1a-tetR-T2A-iRFP

    Plasmid

    #176028

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    iRFP
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHisAlix

    Plasmid

    #42576

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    ALIX (PDCD6IP Human)
    

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pIND-CLucZ

    Plasmid

    #53224

    Purpose

    Inducible expression of Cypridina luciferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Cypridina luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 26, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pFastBac(His10-SNAP-Csk)

    Plasmid

    #177895

    Purpose

    Baculo expression of His10 tag fused to SNAPtag and human Csk

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Csk (CSK Human)
    

    Tags

    10xHis-TEV-TVMV-SNAP tag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-ATG13

    Plasmid

    #171413

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ATG13 (ATG13 Synthetic)
    

    Expression

    Mammalian

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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